从半合成文库中筛选人抗半抗原抗体。

Selection of human anti-hapten antibodies from semisynthetic libraries.

作者信息

Barbas C F, Amberg W, Simoncsits A, Jones T M, Lerner R A

机构信息

Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037.

出版信息

Gene. 1993 Dec 27;137(1):57-62. doi: 10.1016/0378-1119(93)90251-w.

DOI:10.1016/0378-1119(93)90251-w

PMID:8282201

Abstract

Semisynthetic human Fab libraries were constructed, displayed on the surface of filamentous phage and selected for binding to three hapten conjugates. A number of Fabs were isolated and characterized with respect to affinity and specificity. Fabs exhibited affinities of between 80 and 29 nM, as determined by surface plasmon resonance, for the conjugate on which they were selected. Conservation of Asp101 in the third heavy-chain complementarity determining region (HCDR3) appears to be important in the construction of synthetically diverse repertoires.

摘要

构建了半合成人源Fab文库，将其展示在丝状噬菌体表面，并筛选与三种半抗原缀合物结合的文库。分离出了一些Fab，并对其亲和力和特异性进行了表征。通过表面等离子体共振测定，Fab对其筛选所用的缀合物的亲和力在80至29 nM之间。在构建合成多样性文库时，重链第三互补决定区（HCDR3）中Asp101的保守性似乎很重要。