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重组抗体的产生

Generation of recombinant antibodies.

作者信息

Kipriyanov S M, Little M

机构信息

Recombinant Antibody Research Group, German Cancer Research Center, Heidelberg, Germany.

出版信息

Mol Biotechnol. 1999 Sep;12(2):173-201. doi: 10.1385/MB:12:2:173.

DOI:10.1385/MB:12:2:173
PMID:10596374
Abstract

Recombinant antibody technology is opening new perspectives for the development of novel therapeutic and diagnostic agents. In this review we focus on advances in the generation of both genetically engineered humanized and fully human monoclonal antibodies. Methods for their production in different expression systems are also discussed.

摘要

重组抗体技术为新型治疗和诊断试剂的开发开辟了新的前景。在本综述中，我们重点关注基因工程人源化和全人源单克隆抗体生成方面的进展。还讨论了在不同表达系统中生产它们的方法。

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Generation of recombinant antibodies.重组抗体的产生
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本文引用的文献

1
Phage Display.噬菌体展示
Chem Rev. 1997 Apr 1;97(2):391-410. doi: 10.1021/cr960065d.
2
High-level production and long-term storage of engineered antibodies in transgenic tobacco seeds.在转基因烟草种子中高效生产和长期储存工程抗体。
Biotechnology (N Y). 1995 Oct;13(10):1090-3. doi: 10.1038/nbt1095-1090.
3
Epitopes fused to F-pilin are incorporated into functional recombinant pili.与F菌毛蛋白融合的表位被整合到功能性重组菌毛中。
Translating basic mechanisms of IgG effector activity into next generation cancer therapies.
将IgG效应子活性的基本机制转化为下一代癌症疗法。
Cancer Immun. 2012;12:13. Epub 2012 May 1.
4
A novel platform to produce human monoclonal antibodies: The next generation of therapeutic human monoclonal antibodies discovery.一种新型的人源单克隆抗体生产平台：治疗性人源单克隆抗体发现的下一代技术。
MAbs. 2011 Mar-Apr;3(2):203-8. doi: 10.4161/mabs.3.2.14774. Epub 2011 Mar 1.
5
Potential aggregation prone regions in biotherapeutics: A survey of commercial monoclonal antibodies.生物治疗药物中潜在的易于聚集区域：对市售单克隆抗体的一项调查。
MAbs. 2009 May-Jun;1(3):254-67. doi: 10.4161/mabs.1.3.8035. Epub 2009 May 29.
6
Tetrafluorophenolate of HBED-CC: a versatile conjugation agent for 68Ga-labeled small recombinant antibodies.HBED-CC的四氟苯酚盐：一种用于68Ga标记的小型重组抗体的多功能偶联剂。
Eur J Nucl Med Mol Imaging. 2008 Oct;35(10):1878-86. doi: 10.1007/s00259-008-0816-z. Epub 2008 May 29.
7
Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions.具有有效免疫调节功能的人源化抗人CD3单克隆抗体12F6的构建与表征
Immunology. 2005 Dec;116(4):487-98. doi: 10.1111/j.1365-2567.2005.02247.x.
8
Generation and production of engineered antibodies.工程抗体的产生与制备。
Mol Biotechnol. 2004 Jan;26(1):39-60. doi: 10.1385/MB:26:1:39.
9
The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi.酵母和丝状真菌产生抗体片段及抗体融合蛋白。
Microb Cell Fact. 2003 Jan 30;2(1):1. doi: 10.1186/1475-2859-2-1.
J Mol Biol. 1998 Jun 12;279(3):589-603. doi: 10.1006/jmbi.1998.1773.
4
Antibody-IL-12 fusion proteins are effective in SCID mouse models of prostate and colon carcinoma metastases.抗体 - 白细胞介素 - 12融合蛋白在前列腺癌和结肠癌转移的严重联合免疫缺陷（SCID）小鼠模型中具有疗效。
J Immunol. 1998 Jun 15;160(12):6195-203.
5
Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria.靶向内质网、细胞质和线粒体的单链Fv片段的氧化还原状态。
Biotechnology (N Y). 1995 Oct;13(10):1110-5. doi: 10.1038/nbt1095-1110.
6
Eukaryotic virus display: engineering the major surface glycoprotein of the Autographa californica nuclear polyhedrosis virus (AcNPV) for the presentation of foreign proteins on the virus surface.真核病毒展示：改造苜蓿银纹夜蛾核型多角体病毒（AcNPV）的主要表面糖蛋白，用于在病毒表面展示外源蛋白。
Biotechnology (N Y). 1995 Oct;13(10):1079-84. doi: 10.1038/nbt1095-1079.
7
Surface expression and ligand-based selection of cDNAs fused to filamentous phage gene VI.丝状噬菌体基因VI融合cDNA的表面表达及基于配体的筛选
Biotechnology (N Y). 1995 Apr;13(4):378-82. doi: 10.1038/nbt0495-378.
8
Expression of thioredoxin random peptide libraries on the Escherichia coli cell surface as functional fusions to flagellin: a system designed for exploring protein-protein interactions.硫氧还蛋白随机肽文库在大肠杆菌细胞表面作为鞭毛蛋白的功能性融合蛋白表达：一种用于探索蛋白质-蛋白质相互作用的系统。
Biotechnology (N Y). 1995 Apr;13(4):366-72. doi: 10.1038/nbt0495-366.
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Generation of rabbit monoclonal antibody fragments from a combinatorial phage display library and their production in the yeast Pichia pastoris.从组合噬菌体展示文库中产生兔单克隆抗体片段及其在毕赤酵母中的表达
Biotechnology (N Y). 1995 Mar;13(3):255-60. doi: 10.1038/nbt0395-255.
10
Phage diabody repertoires for selection of large numbers of bispecific antibody fragments.用于筛选大量双特异性抗体片段的噬菌体双体文库。
Nat Biotechnol. 1996 Sep;14(9):1149-54. doi: 10.1038/nbt0996-1149.