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Isolation of suppressors of temperature-sensitive folding mutations.

作者信息

Villafane R, Fleming A, Haase-Pettingell C

机构信息

Department of Microbiology, University of Tennessee, Knoxville 37996.

出版信息

J Bacteriol. 1994 Jan;176(1):137-42. doi: 10.1128/jb.176.1.137-142.1994.

DOI:10.1128/jb.176.1.137-142.1994
PMID:8282689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205024/
Abstract

Mutations in the tailspike gene (gene 9) of Salmonella typhimurium phage P22 have been used to identify amino acid interactions during the folding of a polypeptide chain. Since temperature-sensitive folding (tsf) mutations cause folding defects in the P22 tailspike polypeptide chain, it is likely that mutants derived from these and correcting the original tsf defects (second-site intragenic suppressors) identify interactions during the folding pathway. We report the isolation and identification of second-site revertants to tsf mutants.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9f3/205024/9a240b2cdfcd/jbacter00019-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9f3/205024/9a240b2cdfcd/jbacter00019-0163-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9f3/205024/9a240b2cdfcd/jbacter00019-0163-a.jpg

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引用本文的文献

1
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FASEB J. 1996 Jan;10(1):57-66. doi: 10.1096/fasebj.10.1.8566549.

本文引用的文献

1
in vitro MORPHOGENESIS OF PHAGE P22 FROM HEADS AND BASE-PLATE PARTS.噬菌体P22头部和基板部分的体外形态发生
Proc Natl Acad Sci U S A. 1967 Feb;57(2):284-91. doi: 10.1073/pnas.57.2.284.
2
Temperature-sensitive mutants blocked in the folding or subunit of the bacteriophage P22 tail spike protein. II. Active mutant proteins matured at 30 degrees C.在噬菌体P22尾刺蛋白折叠或亚基组装过程中受阻的温度敏感突变体。II. 在30摄氏度下成熟的活性突变蛋白。
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Maturation of the tail spike endorhamnosidase of Salmonella phage P22.
沙门氏菌噬菌体P22尾刺内鼠李糖苷酶的成熟
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4
Temperature-sensitive mutants blocked in the folding or subunit assembly of the bacteriophage P22 tail-spike protein. I. Fine-structure mapping.在噬菌体P22尾刺蛋白折叠或亚基组装过程中受阻的温度敏感突变体。I. 精细结构图谱分析。
Genetics. 1980 Oct;96(2):331-52. doi: 10.1093/genetics/96.2.331.
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Structure and functions of the bacteriophage P22 tail protein.噬菌体P22尾蛋白的结构与功能
J Virol. 1980 Apr;34(1):234-43. doi: 10.1128/JVI.34.1.234-243.1980.
6
Ion-pairs in proteins.蛋白质中的离子对。
J Mol Biol. 1983 Aug 25;168(4):867-85. doi: 10.1016/s0022-2836(83)80079-5.
7
Single amino acid substitutions influencing the folding pathway of the phage P22 tail spike endorhamnosidase.影响噬菌体P22尾刺内鼠李糖苷酶折叠途径的单氨基酸取代
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6584-8. doi: 10.1073/pnas.81.21.6584.
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New M13 vectors for cloning.用于克隆的新型M13载体。
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9
Phage P22 tail protein: gene and amino acid sequence.噬菌体P22尾蛋白：基因与氨基酸序列
Biochemistry. 1982 Nov 9;21(23):5811-5. doi: 10.1021/bi00266a014.
10
A salt bridge stabilizes the helix formed by isolated C-peptide of RNase A.盐桥稳定了由核糖核酸酶A的分离C肽形成的螺旋结构。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2470-4. doi: 10.1073/pnas.79.8.2470.