在不同底物上生长的巴氏甲烷八叠球菌中，480千道尔顿类咕啉蛋白的体外甲基化和合成差异。
Differential in vitro methylation and synthesis of the 480-kilodalton corrinoid protein in Methanosarcina barkeri grown on different substrates.
作者信息
Kremer J, Burchfield S, Frazier C, Krzycki J
机构信息
Department of Microbiology, Ohio State University, Columbus 43212.
出版信息
J Bacteriol. 1994 Jan;176(1):253-5. doi: 10.1128/jb.176.1.253-255.1994.
Abstract
The 480-kDa corrinoid protein was significantly methylated in extracts of acetate- but not methanol-grown cells incubated with 14CH3OH, in part because of its decreased synthesis in cells grown on substrates other than acetate. In addition, a 200-kDa corrinoid protein was methylated in extracts of methanol- but not acetate-grown cells.
摘要
在与¹⁴CH₃OH一起孵育的醋酸盐培养而非甲醇培养的细胞提取物中，480 kDa的类咕啉蛋白发生了显著甲基化，部分原因是其在以醋酸盐以外的底物生长的细胞中合成减少。此外，200 kDa的类咕啉蛋白在甲醇培养而非醋酸盐培养的细胞提取物中发生了甲基化。
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