甲烷八叠球菌属菌株Gö1的N5-甲基-四氢甲蝶呤：辅酶M甲基转移酶是一种可转运钠离子的膜蛋白。
N5-methyl-tetrahydromethanopterin:coenzyme M methyltransferase of Methanosarcina strain Gö1 is an Na(+)-translocating membrane protein.
作者信息
Becher B, Müller V, Gottschalk G
机构信息
Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Germany.
出版信息
J Bacteriol. 1992 Dec;174(23):7656-60. doi: 10.1128/jb.174.23.7656-7660.1992.
Abstract
To determine the cellular localization of components of the methyltransferase system, we separated cell extracts of Methanosarcina strain Gö1 into cytoplasmic and inverted-vesicle fractions. Measurements demonstrated that 83% of the methylene-tetrahydromethanopterin reductase activity resided in the cytoplasm whereas 88% of the methyl-tetrahydromethanopterin:coenzyme M methyltransferase (methyltransferase) was associated with the vesicles. The activity of the methyltransferase was stimulated 4.6-fold by ATP and 10-fold by ATP plus a reducing agent [e.g., Ti(III)]. In addition, methyltransferase activity depended on the presence of Na+ (apparent Km = 0.7 mM) and Na+ was pumped into the lumen of the vesicles in the course of methyl transfer from methyl-tetrahydromethanopterin not only to coenzyme M but also to hydroxycobalamin. Both methyl transfer reactions were inhibited by 1-iodopropane and reconstituted by illumination. A model for the methyl transfer reactions is presented.
摘要
为了确定甲基转移酶系统各组分的细胞定位，我们将甲烷八叠球菌菌株Gö1的细胞提取物分离为细胞质组分和内翻囊泡组分。测量结果表明，83%的亚甲基四氢甲蝶呤还原酶活性存在于细胞质中，而88%的甲基四氢甲蝶呤：辅酶M甲基转移酶（甲基转移酶）与囊泡相关。ATP可使甲基转移酶的活性提高4.6倍，ATP加还原剂[如Ti(III)]可使其活性提高10倍。此外，甲基转移酶活性依赖于Na+的存在（表观Km = 0.7 mM），并且在甲基从甲基四氢甲蝶呤向辅酶M以及向羟基钴胺素转移的过程中，Na+被泵入囊泡腔中。这两种甲基转移反应均受到1-碘丙烷的抑制，并可通过光照进行重构。本文提出了一个甲基转移反应的模型。
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