Yoshimi R, Takamura H, Takasaki K, Tsurumoto H, Kumagami H
Department of Otolaryngology, School of Medicine, Nagasaki University.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Nov;96(11):1922-5. doi: 10.3950/jibiinkoka.96.1922.
It is well known that nasal polyps frequently develop in patients with aspirin-induced asthma, which is thought to be a non-atopic disease. We immunohistochemically examined the eosinophils infiltrating the nasal polyps in patients with aspirin-induced asthma. It has also been established that the monoclonal antibody EG1 reacts with both stored and secretion type ECP (eosinophil cationic protein), and that EG2 reacts only with the secretion type of ECP. EG2 positive cells may thus be considered to be activated eosinophils. Seven cases with nasal polyps associated with aspirin-induced asthma participated in our study. Blood eosinophilia was observed in all 7 cases, but 6 showed normal blood values IgE. Two reacted to house dust antigen, on skin tests and RAST. In the nasal polyps, many EG2 positive cells were observed. On serial sections, the number and distribution of EG1 and EG2 positive cells were almost equal. In the superficial lamina propria, extra-cellular release of ECP was noted. However, epithelial damage did not relate to the number of EG2 positive cells or the degree of extra-cellular EG2 release. In the deep lamina propria, slight extra-cellular ECP release was found. From these results, the eosinophils in nasal polyps accompanying aspirin-induced asthma were thought to be activated. Eosinophils seem to play an important role in the development of nasal polyps. However, tissue injury induced by eosinophils, was not be demonstrated in this study.
众所周知，鼻息肉常见于阿司匹林诱发哮喘的患者，后者被认为是一种非特应性疾病。我们采用免疫组化方法检查了阿司匹林诱发哮喘患者鼻息肉中的嗜酸性粒细胞浸润情况。另外，已经证实单克隆抗体EG1可与储存型和分泌型嗜酸性粒细胞阳离子蛋白（ECP）发生反应，而EG2仅与分泌型ECP发生反应。因此，EG2阳性细胞可被视为活化的嗜酸性粒细胞。7例伴有阿司匹林诱发哮喘的鼻息肉患者参与了我们的研究。所有7例患者均观察到血液嗜酸性粒细胞增多，但6例患者血液IgE值正常。2例患者皮肤试验和放射变应原吸附试验对屋尘抗原有反应。在鼻息肉中，观察到许多EG2阳性细胞。在连续切片上，EG1和EG2阳性细胞的数量和分布几乎相等。在浅层固有层，观察到ECP的细胞外释放。然而，上皮损伤与EG2阳性细胞数量或细胞外EG2释放程度无关。在深层固有层，发现轻微的细胞外ECP释放。根据这些结果，认为阿司匹林诱发哮喘伴发鼻息肉中的嗜酸性粒细胞被激活。嗜酸性粒细胞似乎在鼻息肉的发生发展中起重要作用。然而，本研究未证实嗜酸性粒细胞诱导的组织损伤。
