Min Y G, Kim Y J, Yun Y S
Department of Otorhinolaryngology, College of Medicine, Seoul National University, Korea.
ORL J Otorhinolaryngol Relat Spec. 1996 Mar-Apr;58(2):82-6. doi: 10.1159/000276804.
Nasal polyposis is characterized by heavy eosinophilic infiltration into nasal polyp tissue. Nasal polyps have a predilection for the anteroinferior aspect of the middle turbinate and middle meatal area. To elucidate the pathogenesis of nasal polyps, the authors investigated the distribution of eosinophil infiltrating into nasal polyp tissue, especially at its pedicle, in comparison with the apparently normal nasal mucosa of the middle and inferior turbinates of the same patients. Tissue samples were taken from 12 allergic rhinitis patients with nasal polyps during endoscopic sinus surgery. Three kinds of monoclonal antibodies, EG1, EG2 and BMK-13, were used for immunohistochemical staining. The average number per high-power field of EG1+ cells was 6.33 at the pedicle of polyps and 4.68 and 4.36 at the middle and inferior turbinates, respectively; for EG2+ cells, it was 6.16 at the polyp pedicle and 2.06 and 2.47 at the middle and inferior turbinates, and for BMK-13+ cells, 4.20 at the polyp pedicle and 5.17 and 4.81 at the middle and inferior turbinates. There were no significant differences in the distribution of EG1+ and BMK-13+ cells, but a relatively larger number of activated eosinophils (EG2+ cells) was noted at the pedicle of polyps compared with the other sites (p < 0.025). In addition, the EG2+ cell/EG1+ cell ratio was significantly higher at the pedicle of polyps compared with the other sites (p < 0.01). The results of this study suggest that the activation of eosinophils is significant in the pathogenesis of nasal polyps in atopic patients rather than the number of eosinophils.
鼻息肉的特征是嗜酸性粒细胞大量浸润鼻息肉组织。鼻息肉好发于中鼻甲和中鼻道区域的前下方。为阐明鼻息肉的发病机制，作者研究了嗜酸性粒细胞浸润鼻息肉组织的分布情况，尤其是其蒂部，并与同一患者中、下鼻甲表面看似正常的鼻黏膜进行比较。在内镜鼻窦手术期间，从12例患有鼻息肉的变应性鼻炎患者身上采集组织样本。使用三种单克隆抗体EG1、EG2和BMK - 13进行免疫组织化学染色。息肉蒂部每高倍视野EG1 +细胞的平均数量为6.33，中鼻甲和下鼻甲分别为4.68和4.36；对于EG2 +细胞，息肉蒂部为6.16，中鼻甲和下鼻甲分别为2.06和2.47；对于BMK - 13 +细胞，息肉蒂部为4.20，中鼻甲和下鼻甲分别为5.17和4.81。EG1 +和BMK - 13 +细胞的分布没有显著差异，但与其他部位相比，息肉蒂部的活化嗜酸性粒细胞（EG2 +细胞）数量相对较多（p < 0.025）。此外，与其他部位相比，息肉蒂部的EG2 +细胞/EG1 +细胞比值显著更高（p < 0.01）。本研究结果表明，在特应性患者鼻息肉的发病机制中，嗜酸性粒细胞的活化比嗜酸性粒细胞的数量更重要。
