Jiang X, Umemura K
Department of Otorhinolaryngology, Hamamatsu University School of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Nov;96(11):1926-32. doi: 10.3950/jibiinkoka.96.1926.
We investigated functional and histopathological changes in the inner ear circulatory disorder caused by thrombosis of the anterior inferior cerebellar artery (AICA) in rats. The thrombosis was induced by a photochemical reaction between green light and systemic injection of Rose Bengal. During this experiment, auditory brainstem responses (ABR) were recorded and cochlear blood flow (CBF) was measured by a laser doppler flowmeter. Within 5 weeks after the procedure, histopathological changes were studied by light microscopy following the observation of equilibrium dysfunction and ABR recording. CBF was reduced from the baseline in 30.8 +/- 3.4% (mean +/- SE, n = 25) by occlusion of the AICA, ABR was altered in 96% of animals, and equilibrium dysfunction was observed in 77%. When the AICA was not reopened within a short time, severe histopathological changes were frequently observed. There was no significant correlation between equilibrium dysfunction and histopathological changes in the vestibule. It is concluded that this experimental model may be useful for investigating functional and histopathological changes in the ischemic inner ear.
我们研究了大鼠小脑前下动脉（AICA）血栓形成所致内耳循环障碍的功能和组织病理学变化。血栓形成是通过绿光与全身注射孟加拉玫瑰红之间的光化学反应诱导的。在本实验过程中，记录听觉脑干反应（ABR），并用激光多普勒流量计测量耳蜗血流量（CBF）。在该操作后5周内，在观察到平衡功能障碍和记录ABR后，通过光学显微镜研究组织病理学变化。AICA闭塞使CBF从基线降低了30.8 +/- 3.4%（平均值 +/- 标准误，n = 25），96%的动物ABR发生改变，77%观察到平衡功能障碍。当AICA在短时间内未重新开放时，经常观察到严重的组织病理学变化。平衡功能障碍与前庭组织病理学变化之间无显著相关性。结论是，该实验模型可能有助于研究缺血性内耳的功能和组织病理学变化。
