Sánchez-Criado J E, Tébar M, Sánchez A, Gaytán F
Department of Physiology, Faculty of Medicine, University of Córdoba, Spain.
J Reprod Fertil. 1993 Sep;99(1):173-9. doi: 10.1530/jrf.0.0990173.
Administration of antiprogesterone RU486 to female cyclic rats results in blockade of ovulation associated with both a decreased ovulatory release of LH and an increased rate of follicular atresia. These rats also exhibit increased LH:FSH and testosterone:oestradiol ratios in serum during the period of follicular development as well as an increase in serum concentrations of prolactin that can be suppressed by a dopamine agonist. The increase in either prolactin or testosterone concentrations as well as the relative deficiency in FSH might be responsible for the increase in follicular atresia. The present work evaluated the involvement of LH, FSH, prolactin and testosterone in follicular atresia and in blockade of ovulation induced by RU486 in the cyclic rat. Although bromocriptine treatment did not modify the blockade of ovulation induced by RU486, unilateral ovariectomy at metoestrus and anti-androgen flutamide treatment reversed, in part, the effects of RU486 on both follicular development and ovulation. The combined increase in FSH serum concentration during dioestrus induced by unilateral ovariectomy and the treatment with flutamide had no additive effects. Furthermore, treatment with a superovulatory amount of hFSH did not reverse the effects of RU486. Moreover, unilateral ovariectomy halved testosterone serum concentrations and flutamide treatment had no effect on LH and FSH concentrations in RU486-treated rats. It was therefore concluded that androgens play a role, at least in part, in the process of follicular atresia induced by RU486.
给处于发情周期的雌性大鼠注射抗孕酮RU486会导致排卵受阻，这与促黄体生成素（LH）的排卵释放减少以及卵泡闭锁率增加有关。在卵泡发育期间，这些大鼠血清中的LH:FSH和睾酮:雌二醇比值也会升高，同时血清催乳素浓度增加，而多巴胺激动剂可抑制这种增加。催乳素或睾酮浓度的增加以及促卵泡生成素（FSH）的相对缺乏可能是卵泡闭锁增加的原因。本研究评估了LH、FSH、催乳素和睾酮在卵泡闭锁以及RU486诱导的发情周期大鼠排卵受阻过程中的作用。尽管溴隐亭治疗并未改变RU486诱导的排卵受阻情况，但在动情后期进行单侧卵巢切除术以及使用抗雄激素氟他胺治疗，部分逆转了RU486对卵泡发育和排卵的影响。单侧卵巢切除术诱导的间情期FSH血清浓度升高与氟他胺治疗并无相加作用。此外，用超排卵剂量的人促卵泡生成素（hFSH）治疗并不能逆转RU486的作用。而且，单侧卵巢切除术使RU486处理大鼠的血清睾酮浓度减半，氟他胺治疗对LH和FSH浓度没有影响。因此得出结论，雄激素至少在一定程度上参与了RU486诱导的卵泡闭锁过程。
