Donald P R, Victor T C, Jordaan A M, Schoeman J F, van Helden P D
Department of Paediatrics and Child Health, University of Stellenbosch, Tygerberg, Republic of South Africa.
Scand J Infect Dis. 1993;25(5):613-7. doi: 10.3109/00365549309008550.
43 cerebrospinal fluid (CSF) specimens obtained from 20 children with tuberculous meningitis (TBM) at varying times during the first month of treatment were examined by polymerase chain reaction (PCR) for the presence of M. tuberculosis DNA. Overall 27 CSF specimens (63%) from 16 patients (80%) gave > or = 1 positive results and positive results were obtained from CSF specimens throughout the first 4 weeks of therapy. Nine CSF specimens (21%) gave a doubtful result (only 1 of duplicate determinations positive) and 7 (16%) a negative result. CSF from patients with suspected TBM should be submitted for PCR evaluation and positive results may be obtained up to at least 4 weeks after the start of treatment.
对20例结核性脑膜炎（TBM）患儿在治疗首月不同时间采集的43份脑脊液（CSF）标本进行聚合酶链反应（PCR）检测，以确定是否存在结核分枝杆菌DNA。总体而言，16例患者（80%）的27份CSF标本（63%）检测结果为≥1次阳性，且在治疗的前4周内，CSF标本均获得了阳性结果。9份CSF标本（21%）检测结果可疑（重复检测仅1次阳性），7份（16%）检测结果为阴性。疑似TBM患者的CSF应进行PCR评估，且在治疗开始后至少4周内可能获得阳性结果。
