Hammond A H, Fry J R
Department of Physiology and Pharmacology, Queen's Medical Centre, Nottingham, UK.
Toxicol Lett. 1994 Feb 15;70(3):337-42. doi: 10.1016/0378-4274(94)90128-7.
Omission of serum from culture medium markedly increased the toxicity of the cytochrome P450-activated toxin precocene II in 24-h cultures of rat hepatocytes. In addition, the depletion of glutathione and inhibition of cytochrome P450 in 24-h cultures increased and decreased, respectively, the toxicity of precocene II, as seen in vivo. Precocene II was also toxic to 72-h cultures, although the effect of the serum-free medium was attenuated. However, depletion of glutathione and inhibition of P450 had little or no effect on the toxicity in 72-h cultures. Therefore, the mechanism of precocene II-induced cell death appears to change with time in culture.
在大鼠肝细胞24小时培养中，从培养基中去除血清显著增加了细胞色素P450激活毒素早熟素II的毒性。此外，与体内情况一致，24小时培养中谷胱甘肽的消耗和细胞色素P450的抑制分别增加和降低了早熟素II的毒性。早熟素II对72小时培养也有毒性，尽管无血清培养基的影响减弱。然而，谷胱甘肽的消耗和P450的抑制对72小时培养中的毒性几乎没有影响。因此，早熟素II诱导细胞死亡的机制似乎随培养时间而变化。
