Socha R, Marec F
Institute of Entomology, Czechoslovak Academy of Sciences, Branisovská.
Mutagenesis. 1989 May;4(3):216-20. doi: 10.1093/mutage/4.3.216.
The anti-juvenile hormone agent, precocene II, designated as a prototype of potential fourth-generation insecticides, was subjected to genotoxicity screening by means of the somatic mutation and recombination test in Drosophila melanogaster. Larvae heterozygous for recessive wing trichome mutations, mwh and flr3, were exposed to sublethal concentrations of precocene II, and wings of emerged adult females were inspected for the presence of phenotypically mutant mosaic spots. The compound significantly increased the frequency of mosaic spots in mwh/flr3 wings, but revealed only a slight effect in mwh/TM2 wings. The results suggest that the main sources of genotoxic activity of precocene II are due to chromosome-breakage phenomena resulting from mitotic recombination. The possible mechanism of this effect is discussed.
抗保幼激素剂早熟素II被指定为潜在的第四代杀虫剂原型，通过果蝇的体细胞突变和重组试验进行了遗传毒性筛选。对隐性翅毛状体突变mwh和flr3杂合的幼虫暴露于亚致死浓度的早熟素II，检查羽化成年雌性的翅膀是否存在表型突变镶嵌斑。该化合物显著增加了mwh/flr3翅膀中镶嵌斑的频率，但在mwh/TM2翅膀中仅显示出轻微影响。结果表明，早熟素II遗传毒性活性的主要来源是有丝分裂重组导致的染色体断裂现象。讨论了这种效应的可能机制。
