Sviatchenko V A, Agapov E V, Urmanov I Kh, Serpinskiĭ O I, Frolov I V, Kolykhalov A A, Ryzhikov A B, Netesov S V
Vopr Virusol. 1993 Sep-Dec;38(5):222-6.
A recombinant strain of vaccinia virus (VR26) containing a DNA-copy of the subgenomic 26S RNA of Venezuelan equine encephalomyelitis virus (VEE) inserted into the coding region of thymidine kinase (TK) gene was produced. This subgenomic RNA contained the genes for all structural proteins of the VEE virus, the strain Trinidad donkey (TRD). VR26 effectively expressed VEE virus glycoproteins on the membranes of the infected cells. Blood sera of VR26-immunized animals were found to contain VEE virus-specific antibodies. VR26-immunized mice and rabbits showed a high level of resistance to subcutaneous inoculation with the pathogenic TRD strain of VEE virus. VR26 also provided a high level of protection in animals against aerogenic infection. The absence of virus-neutralizing antibodies in most VR26-immunized animals resistant to inoculation with high doses of VEE suggests the dominant role of the cell component in the immune response. The immune response induced by the recombinant VR26 strain was stable as demonstrated by the resistance of the animals to a challenge with VEE virus 7 months after immunization. The experimental results suggest that this recombinant strain may be considered as a candidate for vaccine preparation.
构建了一种重组痘苗病毒（VR26），其在胸苷激酶（TK）基因的编码区插入了委内瑞拉马脑炎病毒（VEE）亚基因组26S RNA的DNA拷贝。该亚基因组RNA包含了VEE病毒特立尼达驴（TRD）株所有结构蛋白的基因。VR26能在受感染细胞的膜上有效表达VEE病毒糖蛋白。发现用VR26免疫的动物血清中含有VEE病毒特异性抗体。用VR26免疫的小鼠和兔子对皮下接种致病性VEE病毒TRD株表现出高度抗性。VR26还为动物提供了高水平的抵御气溶胶感染的保护。大多数对高剂量VEE接种有抗性的VR26免疫动物中不存在病毒中和抗体，这表明细胞成分在免疫反应中起主导作用。重组VR26株诱导的免疫反应是稳定的，免疫7个月后动物对VEE病毒攻击的抗性证明了这一点。实验结果表明，该重组株可被视为疫苗制备的候选株。
