Zielhorst A J, van Herp F
C R Acad Hebd Seances Acad Sci D. 1976 Dec 20;283(16):1755-8.
Using the alcian blue-alcian yellow staining method, the neurosecretory system in the eyestalk of the Crayfish Astacus leptodactylus is studied during the period following the first, second, third and fourth moult after hatching. On the basis of their staining affinities, six different cell types are distinguishable, always spread over the four optic ganglia and varying in number depending upon the moment of sampling. A clearly recognizable sinus gland is present in the period following the third and fourth moult after hatching. During the successive stages, there occurs a shift in staining pattern of the sinus gland, which is also expressed by the variation in number of the various stained cell types.
采用阿尔辛蓝 - 阿尔辛黄染色法,研究孵化后小龙虾窄指螯虾眼柄中的神经分泌系统在第一次、第二次、第三次和第四次蜕皮后的时期变化。根据它们的染色亲和力,可区分出六种不同的细胞类型,这些细胞总是分布在四个视神经节上,并且数量根据采样时间而变化。在孵化后的第三次和第四次蜕皮后的时期存在一个清晰可辨的窦腺。在连续的阶段中,窦腺的染色模式发生变化,这也通过各种染色细胞类型数量的变化来体现。