Cotta M A
Fermentation Biochemistry Research Unit, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1993 Nov;59(11):3557-63. doi: 10.1128/aem.59.11.3557-3563.1993.
The ability of ruminal bacteria to utilize xylooligosaccharides was examined. Xylooligosaccharides were prepared by partially hydrolyzing oat spelt xylan in phosphoric acid. This substrate solution was added (0.2%, wt/vol) to a complex medium containing yeast extract and Trypticase that was inoculated with individual species of ruminal bacteria, and growth and utilization were monitored over time. All of the xylanolytic bacteria examined were able to utilize this oligosaccharide mixture as a growth substrate. Butyrivibrio fibrisolvens, Eubacterium ruminantium, and Ruminococcus albus used xylooligosaccharides and whole, unhydrolyzed xylan to similar extents, while Prevotella ruminicola used twice as much xylooligosaccharides as xylan (76 versus 34%). Strains of Selenomonas ruminantium were the only nonxylanolytic species that were able to grow on xylooligosaccharides. The ability of individual S. ruminantium strains to utilize xylooligosaccharides was correlated with the presence of xylosidase and arabinosidases activities.
对瘤胃细菌利用低聚木糖的能力进行了检测。低聚木糖是通过在磷酸中部分水解燕麦麸木聚糖制备的。将该底物溶液（0.2%，重量/体积）添加到含有酵母提取物和胰蛋白胨的复合培养基中，该培养基接种了瘤胃细菌的各个种类，并随时间监测其生长和利用情况。所有检测的木聚糖分解细菌都能够利用这种低聚糖混合物作为生长底物。溶纤维丁酸弧菌、反刍真杆菌和白色瘤胃球菌对低聚木糖和完整未水解木聚糖的利用程度相似，而栖瘤胃普雷沃氏菌利用的低聚木糖是木聚糖的两倍（分别为76%和34%）。反刍月形单胞菌菌株是唯一能够在低聚木糖上生长的非木聚糖分解菌。反刍月形单胞菌各个菌株利用低聚木糖的能力与木糖苷酶和阿拉伯糖苷酶活性的存在相关。
