Cole R A, Lindsay D S, Dubey J P, Blagburn B L
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849-5519.
J Vet Diagn Invest. 1993 Oct;5(4):579-84. doi: 10.1177/104063879300500413.
A murine monoclonal antibody (MAb 6G7), isotype IgG2a, produced against tachyzoites of Neospora caninum (isolate NC-1) reacted specifically with tachyzoites of N. caninum in an indirect immunofluorescent antibody test. MAb 6G7 did not react with tachyzoites of Toxoplasma gondii, sporozoites of Isospora suis, Eimeria bovis, or E. tenella, or merozoites of E. bovis in the indirect immunofluorescent antibody test. MAb 6G7 reacted positively with both tachyzoites and bradyzoites of N. caninum in an avidin-biotin peroxidase complex immunohistochemical test on formalin-fixed paraffin-embedded tissues. No reaction was observed with the following: tachyzoites and bradyzoites of T. gondii, T. gondii-like parasites, or Hammondia hammondi; bradyzoites of Frenkelia microti; schizonts and merozoites of Sarcocystis-like organisms; schizonts, sarcocysts, and oocysts/sporocysts of S. cruzi; schizonts and merozoites of S. canis; schizonts of S. hirsuta, S. tenella, and S. capracanis; merozoites of S. neurona and S. neurona-like organisms, E. bovis, or Haemoproteus sp.; bradyzoites and merozoites of S. montanaensis; bradyzoites of S. odocoileocanis, S. cruzi, and S. tenella; meronts, sexual stages, and caryocysts of Caryospora sp. and C. bigenetica; micromerozoites, macromerozoites, and schizonts of Hepatozoon canis; sporozoites, sexual stages, and oocysts of Cryptosporidium parvum and C. baileyi; trophozoites of Monocystis lumbrici, Tritrichomonas foetus, and Balantidium coli; tissue cysts and bradyzoites of Besnoitia sp. and B. jellisoni; amastigotes of Leishmania sp.; and trophic theronts of Ichthyopthirius multifilis. MAb 6G7 reacted with tachyzoites and bradyzoites of N. caninum in natural and experimental infections in dogs, cattle, mice, rats, sheep, and goats, indicating that host origin of the tissues did not affect the performance of the test.
一种针对犬新孢子虫（分离株NC-1）速殖子产生的鼠单克隆抗体（MAb 6G7），同种型为IgG2a，在间接免疫荧光抗体试验中与犬新孢子虫速殖子发生特异性反应。在间接免疫荧光抗体试验中，MAb 6G7不与刚地弓形虫速殖子、猪等孢球虫子孢子、牛艾美耳球虫、柔嫩艾美耳球虫或牛艾美耳球虫裂殖子发生反应。在对福尔马林固定石蜡包埋组织进行的抗生物素蛋白-生物素过氧化物酶复合物免疫组织化学试验中，MAb 6G7与犬新孢子虫的速殖子和缓殖子均呈阳性反应。未观察到与以下病原体的反应：刚地弓形虫的速殖子和缓殖子、类刚地弓形虫寄生虫或哈蒙德氏球虫；微小弗氏球虫的缓殖子；类肉孢子虫的裂殖体和裂殖子；克鲁斯肉孢子虫的裂殖体、肉孢子囊和卵囊/子孢子囊；犬肉孢子虫的裂殖体和裂殖子；毛肉孢子虫、柔嫩艾美耳球虫和山羊艾美耳球虫的裂殖体；新孢子虫和类新孢子虫、牛艾美耳球虫或变形血原虫的裂殖子；蒙大拿肉孢子虫的缓殖子和裂殖子；奥氏肉孢子虫、克鲁斯肉孢子虫和柔嫩艾美耳球虫的缓殖子；卡里孢子虫属和双基因卡里孢子虫的裂殖体、有性阶段和包囊；犬肝簇虫的微小裂殖子、大裂殖子和裂殖体；微小隐孢子虫和贝氏隐孢子虫的子孢子、有性阶段和卵囊；蚯蚓单囊虫、胎儿三毛滴虫和结肠小袋纤毛虫的滋养体；贝斯诺球虫属和杰利森贝斯诺球虫的组织包囊和缓殖子；利什曼原虫的无鞭毛体；以及多子小瓜虫的营养型游动孢子。MAb 6G7在犬、牛、小鼠、大鼠、绵羊和山羊的自然感染和实验感染中与犬新孢子虫的速殖子和缓殖子发生反应，表明组织的宿主来源不影响试验结果。
