Abbasoglu O, Sayek I, Hasçelik G
Department of Surgery, Hacettepe University School of Medicine, Ankara, Turkey.
Eur J Surg. 1993 Oct;159(10):521-4.
To assess the effect of povidone iodine lavage on peritoneal defence mechanisms in rats.
Randomised study.
90 Wistar albino rats.
Rats were divided in three groups of 30. All rats underwent midline laparotomy, and 20 rats in each group had peritoneal lavage with 20 ml 1% povidone iodine, samples being taken before lavage in 10 and after lavage in 10.
Effect of povidone-iodine lavage compared with laparotomy alone on bactericidal activity of peritoneal fluid (group 1); chemotactic indices of polymorphonuclear leucocytes (PMNL) (group 2); and total cell counts, cell types, and peritoneal phagocytic activity (group 3).
Lavage with povidone-iodine increased the total peritoneal cell count/washing from 10.4 x 10(6) to 12.4 x 10(6) (p < 0.005), peritoneal bactericidal activity from 69.2% to 92.7% (p < 0.05), and peritoneal phagocytic activity from 58.9% to 73.7% (p < 0.05) at the end of four hours. Chemotactic indices of peritoneal PMNL before and after lavage were 1.90 and 1.89, respectively. Among the cells in the peritoneal fluid, the proportion of PMNL increased, whereas those of macrophages, lymphocytes, and mast cells decreased (p < 0.005).
These results differ from those that we found previously after saline lavage. Lavage with 1% povidone-iodine does not increase the detrimental effect of saline lavage; on the contrary, it may even enhance peritoneal defence mechanisms. Any detrimental effects of povidone-iodine lavage should be attributed to systemic rather than local toxicity.
评估聚维酮碘灌洗对大鼠腹膜防御机制的影响。
随机研究。
90只Wistar白化大鼠。
将大鼠分为三组，每组30只。所有大鼠均接受正中剖腹术，每组20只大鼠用20毫升1%聚维酮碘进行腹膜灌洗，10只在灌洗前取样，10只在灌洗后取样。
与单纯剖腹术相比，聚维酮碘灌洗对腹膜液杀菌活性的影响（第1组）；多形核白细胞（PMNL）的趋化指数（第2组）；以及细胞总数、细胞类型和腹膜吞噬活性（第3组）。
聚维酮碘灌洗使4小时末腹膜总细胞计数/冲洗液从10.4×10⁶增加到12.4×10⁶（p<0.005），腹膜杀菌活性从69.2%提高到92.7%（p<0.05），腹膜吞噬活性从58.9%提高到73.7%（p<0.05）。灌洗前后腹膜PMNL的趋化指数分别为1.90和1.89。在腹膜液中的细胞中，PMNL的比例增加，而巨噬细胞、淋巴细胞和肥大细胞的比例下降（p<0.005）。
这些结果与我们之前在生理盐水灌洗后发现的结果不同。1%聚维酮碘灌洗不会增加生理盐水灌洗的有害作用；相反，它甚至可能增强腹膜防御机制。聚维酮碘灌洗的任何有害作用应归因于全身毒性而非局部毒性。
