Whitten P L, Lewis C, Naftolin F
Department of Anthropology, Emory University, Atlanta, Georgia 30322.
Biol Reprod. 1993 Nov;49(5):1117-21. doi: 10.1095/biolreprod49.5.1117.
The effects of a phytoestrogen diet on sexual differentiation were examined in lactationally exposed rat pups. Rat dams were provided a semipurified diet containing the isoflavonoid coumestrol at a concentration (0.01%) previously found to be uterotrophic. Coumestrol treatment did not significantly alter the time of vaginal opening, although vaginal opening did occur at a lighter body weight. By 132 days of age, 83% of coumestrol-treated females exhibited the cornified smears of a persistent estrous state. By contrast, 91% of control animals were cycling regularly at 132 days of age. Estradiol stimulation failed to elicit an LH elevation in the coumestrol-treated animals, suggesting the possibility of neuroendocrine impairments. These findings indicate that the female offspring of mothers fed a low-level phytoestrogen diet during lactation manifest early and nearly universal disruption of cyclicity of the persistent-estrus type.
在哺乳期接触过植物雌激素饮食的幼鼠中，研究了植物雌激素饮食对性别分化的影响。给孕鼠提供一种半纯化饮食，其中含有异黄酮香豆雌酚，其浓度（0.01%）先前已被发现具有子宫营养作用。香豆雌酚处理并未显著改变阴道开口的时间，不过阴道开口确实在体重较轻时就出现了。到132日龄时，83%接受香豆雌酚处理的雌性呈现出持续发情状态的角化涂片。相比之下，91%的对照动物在132日龄时正常发情。在接受香豆雌酚处理的动物中，雌二醇刺激未能引发促黄体生成素升高，这表明存在神经内分泌损伤的可能性。这些发现表明，哺乳期食用低水平植物雌激素饮食的母鼠所产雌性后代，早期几乎普遍出现持续性发情类型的发情周期紊乱。
