Jackson J A, Zhang P, Bahr J M
Department of Animal Sciences, University of Illinois, Urbana 61801.
Biol Reprod. 1993 Nov;49(5):1141-6. doi: 10.1095/biolreprod49.5.1141.
The stages of follicular maturation of a preovulatory follicle in the chicken can be divided into an extended proliferative phase (prior to the LH surge) and a brief ovulatory phase (after the LH surge). There is evidence to suggest that plasminogen activator (PA) may play a role in follicular maturation and ovulation in the chicken. The objective of this study was to determine whether PA activity changes in the stigma (site of follicular rupture) and nonstigma regions of the theca layer, granulosa layer, and germinal disc region (germinal disc and adjacent granulosa cells; GDR) during the transition from the proliferative phase to the ovulatory phase and immediately before ovulation. The largest preovulatory follicle (F1) and the second-largest follicle (F2), which would have ovulated first and 26 h later, respectively, were collected at 8 h and 2 h before ovulation (before and after the LH surge, respectively), and at 0.5 h (immediately before ovulation). PA activity in the stigma region of the theca layer in the F1 and F2 follicles was very low at 8 h before ovulation (before the LH surge) and increased dramatically (9-fold) at 2 h before ovulation (after the LH surge). PA activity in the stigma region of the F1 follicles remained high as the follicles prepared to ovulate (0.5 h before ovulation), whereas in the stigma region of the less mature F2 follicles it decreased to a level similar to that measured 8 h before ovulation.(ABSTRACT TRUNCATED AT 250 WORDS)
鸡排卵前卵泡的卵泡成熟阶段可分为一个延长的增殖期（促黄体生成素高峰之前）和一个短暂的排卵阶段（促黄体生成素高峰之后）。有证据表明，纤溶酶原激活物（PA）可能在鸡的卵泡成熟和排卵过程中发挥作用。本研究的目的是确定在从增殖期到排卵阶段的过渡期间以及排卵前即刻，PA活性在卵泡膜层、颗粒层和胚盘区域（胚盘及相邻颗粒细胞；GDR）的柱头（卵泡破裂部位）和非柱头区域是否发生变化。分别在排卵前8小时和2小时（分别在促黄体生成素高峰之前和之后）以及0.5小时（排卵前即刻）收集最大的排卵前卵泡（F1）和第二大卵泡（F2），F1和F2卵泡分别会在26小时后排卵。在排卵前8小时（促黄体生成素高峰之前），F1和F2卵泡卵泡膜层柱头区域的PA活性非常低，而在排卵前2小时（促黄体生成素高峰之后）则急剧增加（9倍）。随着卵泡准备排卵（排卵前0.5小时），F1卵泡柱头区域的PA活性仍然很高，而在不太成熟的F2卵泡柱头区域，其活性降至与排卵前8小时测得的水平相似。（摘要截短于250字）
