Sundaresan N R, Anish D, Saxena V K, Sastry K V H, Jain Preeti, Singh Rani, Saxena M, Ahmed K A
Disease Genetics and Biotechnology Laboratory, Central Avian Research Institute, Izatnagar, 243122 Bareilly, India.
Vet Res Commun. 2008 Aug;32(6):419-26. doi: 10.1007/s11259-007-9025-7. Epub 2007 Nov 9.
In the present experiment, we studied the spatial expression profiles of chemokines and cytokines mRNA in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle in chicken using semi-quantitative PCR. The mRNAs of IL-1beta, IL-6, GM-CSF, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IFN-gamma, IL-4, IL-13, IL-10 and TGF-beta2 were expressed in the granulosa (F1Gr) and theca (F1Th) layers of the largest preovulatory follicle. However, the transcripts of IL-2 were not detected in any of the samples tested. Significantly higher levels of IL-6 and GM-CSF mRNA expression were noticed in F1Gr when compared to F1Th layer. Expression of chCXCLi2, a CXC chemokine, was almost similar in F1Gr and F1Th layers. However, the expression of CCL chemokines i.e. chCCLi2, chCCLi4, chCCLi7 mRNAs were almost 2 folds higher in F1Th layer in comparison to F1Gr layer. The expression of Th2 cytokines (IL-4 and IL-13) mRNA was noticed in F1Gr and F1Th layers with higher levels in the former. Expression of IFN-gamma mRNA was noticed in F1Gr and F1Th layers. Significantly higher level of TGF-beta2 expression was observed in F1Th in comparison to F1Gr layer. It was concluded from the present study that the mRNA expression of cytokines and chemokines are differentially regulated in the granulosa and theca layers of the largest preovulatory follicle in chicken.
在本实验中，我们使用半定量PCR研究了鸡最大排卵前卵泡颗粒层（F1Gr）和卵泡膜层（F1Th）中趋化因子和细胞因子mRNA的空间表达谱。IL-1β、IL-6、GM-CSF、chCXCLi2、chCCLi2、chCCLi4、chCCLi7、IFN-γ、IL-4、IL-13、IL-10和TGF-β2的mRNA在最大排卵前卵泡的颗粒层（F1Gr）和卵泡膜层（F1Th）中均有表达。然而，在所检测的任何样本中均未检测到IL-2的转录本。与F1Th层相比，F1Gr层中IL-6和GM-CSF mRNA表达水平显著更高。CXC趋化因子chCXCLi2在F1Gr层和F1Th层中的表达几乎相似。然而，CCL趋化因子即chCCLi2、chCCLi4、chCCLi7 mRNA在F1Th层中的表达比F1Gr层高近2倍。Th2细胞因子（IL-4和IL-13）mRNA在F1Gr层和F1Th层中均有表达，且在前者中水平更高。IFN-γ mRNA在F1Gr层和F1Th层中均有表达。与F1Gr层相比，F1Th层中TGF-β2的表达水平显著更高。本研究得出结论，鸡最大排卵前卵泡颗粒层和卵泡膜层中细胞因子和趋化因子的mRNA表达受到不同调控。
