Watkins K P, Dungan J M, Agabian N
University of California, San Francisco.
Cell. 1994 Jan 14;76(1):171-82. doi: 10.1016/0092-8674(94)90181-3.
In vivo psoralen cross-linking of the trypanosome spliced leader (SL) RNA has led to the discovery of a small RNA that we provisionally call the spliced leader-associated (SLA) RNA. The 72 nt SLA RNA is unlike any known small RNA except for a small region that resembles U5 snRNA. The SL/SLA RNA cross-links map to two regions, the predominant interactions occurring between the 5' splice site region of the SL RNA and a CUUUUA sequence in the SLA RNA. The resemblance between these cross-links and interactions of U5 snRNA with cis-spliced pre-mRNAs suggests that the SLA RNA may be the trans-splicing analog of U5 snRNA in trypanosomes.
在体内对锥虫剪接前导序列（SL）RNA进行补骨脂素交联，从而发现了一种小RNA，我们暂时将其称为剪接前导序列相关（SLA）RNA。除了一个类似于U5 snRNA的小区域外，这种72个核苷酸的SLA RNA与任何已知的小RNA都不同。SL/SLA RNA交联定位于两个区域，主要的相互作用发生在SL RNA的5'剪接位点区域与SLA RNA中的CUUUUA序列之间。这些交联以及U5 snRNA与顺式剪接前体mRNA的相互作用之间的相似性表明，SLA RNA可能是锥虫中U5 snRNA的反式剪接类似物。
