通过荧光原位杂交将人类PAX4基因定位于染色体7q32带。

Assignment of the human PAX4 gene to chromosome band 7q32 by fluorescence in situ hybridization.

作者信息

Tamura T, Izumikawa Y, Kishino T, Soejima H, Jinno Y, Niikawa N

机构信息

Department of Human Genetics, Nagasaki University School of Medicine, Japan.

出版信息

Cytogenet Cell Genet. 1994;66(2):132-4. doi: 10.1159/000133684.

DOI:10.1159/000133684

PMID:8287686

Abstract

Of the nine known members of a human paired box-containing gene family (Pax), only PAX4 has not been precisely localized. We screened a cosmid library of human genomic DNA using polymerase chain reaction products for PAX4 as a probe and isolated three positive cosmid clones. Sequence analysis revealed that at least two of them had exon-like sequences and showed extensive homology to Pax-4 in the mouse. These two cosmid clones were mapped to human chromosome band 7q32 by fluorescence in situ hybridization.

摘要

在人类含配对盒基因家族（Pax）已知的9个成员中，只有PAX4尚未精确定位。我们以PAX4的聚合酶链反应产物为探针，筛选了人类基因组DNA的黏粒文库，分离出3个阳性黏粒克隆。序列分析显示，其中至少有两个含有外显子样序列，并且与小鼠的Pax - 4具有广泛的同源性。通过荧光原位杂交，将这两个黏粒克隆定位于人类染色体7q32带。