Wreggett K A, Hill F, James P S, Hutchings A, Butcher G W, Singh P B
Department of Development and Signalling, AFRC Babraham Institute, Cambridge, UK.
Cytogenet Cell Genet. 1994;66(2):99-103. doi: 10.1159/000133676.
The Drosophila HP1 gene contains a highly conserved sequence, the chromobox, which can be used to isolate HP1-like genes from both mouse (M31 and M32) and man (HSM1) (Singh et al., 1991). Here we report that a monoclonal antibody (MoAb) raised against the M31 protein recognises a 26-kDa protein in murine and human nuclear extracts and localises to large masses of condensed chromatin within murine interphase nuclei, some of which are associated with the nucleoli. At metaphase, the MoAb binds to the centromeres of both human and murine chromosomes. The evolutionary conservation of this chromosomal localisation indicates that the M31 protein is likely to be important in the packaging of mammalian chromosomal DNA into constitutive heterochromatin.
果蝇HP1基因包含一个高度保守的序列——染色质盒，该序列可用于从小鼠（M31和M32）和人类（HSM1）中分离出类似HP1的基因（辛格等人，1991年）。在此我们报告，一种针对M31蛋白产生的单克隆抗体（MoAb）可识别小鼠和人类核提取物中的一种26 kDa蛋白，并定位于小鼠间期核内大量浓缩染色质团块，其中一些与核仁相关。在中期，该MoAb结合于人类和小鼠染色体的着丝粒。这种染色体定位的进化保守性表明，M31蛋白可能在将哺乳动物染色体DNA包装成组成型异染色质方面起重要作用。
