Horsley D, Hutchings A, Butcher G W, Singh P B
Department of Development and Genetics, Babraham Institute, Cambridge, UK.
Cytogenet Cell Genet. 1996;73(4):308-11. doi: 10.1159/000134363.
Mice possess two structural homologues of Drosophila HP1, termed M31 and M32 (Singh et al., 1991). We have previously shown that an M31-specific monoclonal antibody (MoAb), MAC 353, localises to constitutive heterochromatin (Wreggett et al., 1994). Here we report that a MoAb raised against the M32 protein (MAC 385) recognises a 22-kDa protein in murine nuclear extracts and that M32 is distributed in a fine-grain "speckled" pattern within interphase nuclei. M32 is also largely excluded from the large masses of constitutive heterochromatin that are labelled by MAC 353.
小鼠有两种果蝇HP1的结构同源物，称为M31和M32（辛格等人，1991年）。我们之前已经表明，一种M31特异性单克隆抗体（MoAb），MAC 353，定位于组成型异染色质（雷格特等人，1994年）。在此我们报告，一种针对M32蛋白产生的MoAb（MAC 385）在鼠核提取物中识别一种22 kDa的蛋白，并且M32在间期核内以细颗粒“斑点”模式分布。M32也基本不位于被MAC 353标记的大量组成型异染色质中。
