Xin X Y
Xi Jing Hospital, Fourth Military University, Xi'an.
Zhonghua Fu Chan Ke Za Zhi. 1993 Jul;28(7):405-7, 442.
In this study, [a-32p]dATP labelled probes of c-myc, N-ras and c-erb B genes were employed to perform dot blot hybridisation with DNA extracted from 17 cases of primary ovarian cancer, 3 cases of recurrent ovarian cancer, 2 cases of germ cell ovarian cancer, 5 cases of serous cysadonoma and 4 cases of normal ovarian tissue. Some specimens were examined by Southern blot hybridisation. The results showed varying degrees of amplification: c-myc 54.5%, N-ras 64.1% and c-erb B 31.8%, in the 22 cases of ovarian malignancies. All were significantly higher than in the three benign tumours and in normal ovarian tissue. Frequency of amplification of more than 2 oncogencis at the same time was found to be 40.9%. There was a negative correlation between amplification of c-myc and differentiation. A relatively high amplification of N-ras was present in late stage ovarian cancer. Patients with c-erb B amplification showed a shorter survival period than those without amplification.
在本研究中，采用[a-32P]dATP标记的c-myc、N-ras和c-erb B基因探针，与从17例原发性卵巢癌、3例复发性卵巢癌、2例卵巢生殖细胞癌、5例浆液性囊腺瘤及4例正常卵巢组织中提取的DNA进行斑点杂交。部分标本采用Southern印迹杂交检测。结果显示，在22例卵巢恶性肿瘤中，c-myc、N-ras和c-erb B基因存在不同程度的扩增，分别为54.5%、64.1%和31.8%。均显著高于3种良性肿瘤及正常卵巢组织。同时发现2种以上癌基因扩增的频率为40.9%。c-myc基因扩增与分化呈负相关。N-ras基因在晚期卵巢癌中扩增相对较高。c-erb B基因扩增的患者生存期较未扩增者短。
