Elsaesser F, Bruhn T O, Parvizi N
Institut für Tierzucht und Tierverhalten (FAL), Mariensee, Federal Republic of Germany.
J Reprod Fertil. 1988 Jan;82(1):71-80. doi: 10.1530/jrf.0.0820071.
Monolayer cultures of anterior pituitary cells from male or female pigs of 60, 80, 105 days of fetal life or of 60, 160 and 250 days of post-natal life were prepared and treated with LHRH (1 pM to 10 nM). Dose-related increases of LH were first seen at 80 days of gestation in both sexes, while only female fetuses responded to maximal LHRH at 60 days. Basal and stimulated LH release doubled in cultures from 105-day-old fetuses when compared with those at 80 days. Compared to late fetal stages LH release was 20- to 30-fold higher in cell cultures from 60-day-old (post-natal) donors without further change during the post-natal period. In all pre- and post-natal age groups basal and maximal LH release of pituitary cells from males was lower than that of females. FSH stimulation started in male and female cells at 80 days of gestation only at LHRH concentrations exceeding or equal to 0.1 nM. By 105 days FSH secretion was dose-related and pituitary cells of females responded with higher FSH values than did those of males. In general, post-natal cells released much higher amounts of FSH than did prenatal cells. Basal and maximal release of FSH decreased during post-natal development in both sexes. Basal as well as maximal FSH release of cultures from female donors was higher than that found in cultures from male donors. Determination of total LH and FSH content in fetal pituitary cell cultures indicated that the developmental increase in gonadotrophin release potential is a function of the total gonadotrophin content in vitro. We conclude that (1) the in-vitro release of gonadotrophins to LHRH is dose-, age- and sex-dependent; (2) in the female fetal pig LH responsiveness develops earlier than FSH responsiveness; (3) apparently, these maturational changes mainly reflect alterations in pituitary gonadotrophin content; and (4) there is no simple relationship between in-vitro release and circulating gonadotrophins.
制备了来自胎龄60、80、105天或出生后60、160和250天的雄性或雌性猪的垂体前叶细胞单层培养物，并用促黄体生成素释放激素（LHRH，1皮摩尔至10纳摩尔）进行处理。在两性中，妊娠80天时首次观察到促黄体生成素（LH）呈剂量依赖性增加，而只有雌性胎儿在60天时对最大剂量的LHRH有反应。与80天龄胎儿的培养物相比，105天龄胎儿的培养物中基础和刺激后的LH释放量增加了一倍。与胎儿后期阶段相比，来自60日龄（出生后）供体的细胞培养物中LH释放量高20至30倍，在出生后阶段没有进一步变化。在所有产前和产后年龄组中，雄性垂体细胞的基础和最大LH释放量均低于雌性。仅在LHRH浓度超过或等于0.1纳摩尔时，雄性和雌性细胞在妊娠80天时才开始对促卵泡生成素（FSH）产生刺激反应。到105天时，FSH分泌呈剂量依赖性，雌性垂体细胞的FSH值反应高于雄性。一般来说，产后细胞释放的FSH量比产前细胞多得多。两性产后发育过程中FSH的基础释放量和最大释放量均下降。雌性供体培养物的基础和最大FSH释放量高于雄性供体培养物。对胎儿垂体细胞培养物中总LH和FSH含量的测定表明，促性腺激素释放潜能的发育性增加是体外总促性腺激素含量的函数。我们得出以下结论：（1）促性腺激素在体外对LHRH的释放是剂量、年龄和性别依赖性的；（2）在雌性胎儿猪中，LH反应性比FSH反应性发育得更早；（3）显然，这些成熟变化主要反映了垂体促性腺激素含量的改变；（4）体外释放与循环促性腺激素之间没有简单的关系。
