Giordano M, Takashima H, Herranz A, Poltorak M, Geller H M, Marone M, Freed W J
NIMH-Neuroscience Center, St. Elizabeths, Washington, DC 20032.
Exp Neurol. 1993 Dec;124(2):395-400. doi: 10.1006/exnr.1993.1213.
Embryonic striatal cells were immortalized using the A58 temperature-sensitive allele of the SV40 large T antigen. Two cell lines, M213-2O and M26-1F, with gamma-aminobutyric acid (GABA)ergic properties were selected from 85 clones thus developed. M213-2O is a multipolar, polygonal cell line which expresses SV40 large T antigen and glutamate decarboxylase (GAD) at the permissive temperature (33 degrees C) and GAD and MAP-2 immunoreactivity at the nonpermissive temperature (39.3 degrees C). M26-1F has a fibroblast-like morphology and expresses SV40 large T antigen and GAD both at the permissive and nonpermissive temperatures and MAP-2 immunoreactivity at the nonpermissive temperature. Both lines contain GABA as measured by reversed-phase HPLC and M213-2O expresses nipecotic-sensitive [14C]GABA uptake.
使用SV40大T抗原的A58温度敏感等位基因使胚胎纹状体细胞永生化。从由此培养出的85个克隆中筛选出两个具有γ-氨基丁酸（GABA）能特性的细胞系，即M213-2O和M26-1F。M213-2O是一种多极、多边形细胞系，在允许温度（33摄氏度）下表达SV40大T抗原和谷氨酸脱羧酶（GAD），在非允许温度（39.3摄氏度）下表达GAD和MAP-2免疫反应性。M26-1F具有成纤维细胞样形态，在允许温度和非允许温度下均表达SV40大T抗原和GAD，在非允许温度下表达MAP-2免疫反应性。通过反相高效液相色谱法测定，两个细胞系均含有GABA，且M213-2O表达对哌啶酸敏感的[14C]GABA摄取。
