Ross Karen C, Waldman Barbara C, Conejero-Goldberg Concepcion, Freed William, Coleman James R
Department of Psychology, University of South Carolina, Columbia, South Carolina 29208, USA.
Exp Neurol. 2002 Sep;177(1):338-40. doi: 10.1006/exnr.2002.7987.
The purpose of the present study was to examine the effects of GABA-producing cell transplants on audiogenic seizures (AGS). The M213-2O cell line was derived from fetal rat striatum and has GABAergic properties. This cell line was further modified to express human GAD(67) and produce elevated levels of GABA. The present study compares the effects of parent M213-2O cell transplants with those of GAD(67)-modified M213-2O cells in AGS-prone Long-Evans rats. Two weeks following implantation of engineered cells, latency to AGS-typical wild running was increased compared to nonimplanted subjects. Survival of the transplanted cells was confirmed by immunochemical labeling of GAD(67) and Epstein-Barr virus nuclear antigen. These findings support the use of GABA-producing cell lines to modify seizure activity.
本研究的目的是检验产生γ-氨基丁酸（GABA）的细胞移植对听源性癫痫（AGS）的影响。M213-2O细胞系源自胎鼠纹状体，具有γ-氨基丁酸能特性。该细胞系经过进一步改造，以表达人谷氨酸脱羧酶（GAD）（67）并产生更高水平的GABA。本研究比较了亲代M213-2O细胞移植与经GAD（67）改造的M213-2O细胞移植对易患AGS的Long-Evans大鼠的影响。在植入工程细胞两周后，与未植入细胞的大鼠相比，AGS典型的狂奔潜伏期延长。通过对GAD（67）和EB病毒核抗原进行免疫化学标记，证实了移植细胞的存活。这些发现支持使用产生GABA的细胞系来改变癫痫活动。
