Drent M, van Velzen-Blad H, Diamant M, Wagenaar S S, Hoogsteden H C, van den Bosch J M
Dept of Pulmonary Diseases, St. Antonius Hospital, Nieuwegein, The Netherlands.
Eur Respir J. 1993 Oct;6(9):1276-81.
The aim of the present study was to investigate whether bronchoalveolar lavage (BAL) cell profile and immunoglobulin levels from patients with extrinsic allergic alveolitis (EAA) were related to the time elapsed between last antigen exposure and BAL. For this purpose, an analysis was performed of BAL fluid (BALF) obtained from 59 nonsmoking EAA patients at various time-points after termination of antigen exposure and BAL. BALF early after antigen provocation (group 1: < 24 h) contained high absolute and relative numbers of lymphocytes, neutrophils, eosinophils and mast cells, and a low relative number of alveolar macrophages. When obtained after recent antigen exposure (group 2: 2-7 days), BALF showed high numbers of lymphocytes, plasma cells and mast cells, and high levels of immunoglobulins M, G and A (IgM, IgG and IgA). In BAL obtained one week or more after the final antigen exposure, (Group 3: 8-30 days; Group 4: 1-12 months) the distribution of all constituents showed a tendency to return to normal values, with the exception of the lymphocytes. These results demonstrate that BAL cell profile and immunoglobulin levels in EAA are highly dependent on the time-point at which the material is obtained in relation to the last exposure to the causative antigen.
本研究的目的是调查外源性过敏性肺泡炎（EAA）患者的支气管肺泡灌洗（BAL）细胞谱和免疫球蛋白水平是否与末次抗原暴露至BAL的时间间隔有关。为此，对59例非吸烟EAA患者在终止抗原暴露和BAL后的不同时间点采集的支气管肺泡灌洗液（BALF）进行了分析。抗原激发后早期（第1组：<24小时）的BALF中淋巴细胞、中性粒细胞、嗜酸性粒细胞和肥大细胞的绝对数和相对数较高，而肺泡巨噬细胞的相对数较低。在近期抗原暴露后（第2组：2 - 7天）采集的BALF显示淋巴细胞、浆细胞和肥大细胞数量较多，免疫球蛋白M、G和A（IgM、IgG和IgA）水平较高。在末次抗原暴露一周或更长时间后采集的BAL（第3组：8 - 30天；第4组：1 - 12个月）中，除淋巴细胞外，所有成分的分布均有恢复至正常水平的趋势。这些结果表明，EAA患者的BAL细胞谱和免疫球蛋白水平高度依赖于采集样本时相对于末次接触致病抗原的时间点。
