Crowther R A, Olesen O F, Smith M J, Jakes R, Goedert M
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
FEBS Lett. 1994 Jan 10;337(2):135-8. doi: 10.1016/0014-5793(94)80260-2.
The principal fibrous component of neurofibrillary pathology in Alzheimer's disease, the paired helical filament, is formed from hyperphosphorylated microtubule-associated protein tau. Here we show that recombinant tau protein either in a non-phosphorylated state or following phosphorylation with brain extract can be assembled in vitro into filaments resembling those seen in Alzheimer's disease.
阿尔茨海默病神经原纤维病变的主要纤维成分——双螺旋丝，是由过度磷酸化的微管相关蛋白tau形成的。我们在此表明，无论是处于非磷酸化状态的重组tau蛋白，还是经脑提取物磷酸化后的重组tau蛋白，都能在体外组装成与阿尔茨海默病中所见细丝相似的细丝。
