同嘌呤/同嘧啶序列作为真核细胞中的潜在调控元件。

Homopurine/homopyrimidine sequences as potential regulatory elements in eukaryotic cells.

Lu G, Ferl R J

Horticultural Sciences Department, University of Florida, Gainesville 32611.

Int J Biochem. 1993 Nov;25(11):1529-37. doi: 10.1016/0020-711x(93)90508-c.

Abstract

Homopurine/homopyrimidine (PuPy) repetitive duplex sequences can form intramolecular triplexes (H-DNA) or intermolecular triplexes with a third strand in a sequence-specific manner. 2. Such sequences are present in natural genomes within 5'- and 3'-flanking sequences and coding regions of genes. Triplex DNA structures have been detected in vitro and in vivo and have been immunolocalized to chromosomes by triplex-specific monoclonal antibody approaches. 3. Intermolecular triplex formation represses gene expression at the transcriptional level and is also useful in genomic mapping, gene cloning, sequence-specific drug delivery, and selective modulation of gene expression.

摘要

同嘌呤/同嘧啶（PuPy）重复双链序列能够以序列特异性方式形成分子内三链体（H-DNA）或与第三条链形成分子间三链体。2. 此类序列存在于天然基因组中基因的5'和3'侧翼序列以及编码区域内。三链体DNA结构已在体外和体内被检测到，并且通过三链体特异性单克隆抗体方法已将其免疫定位到染色体上。3. 分子间三链体的形成在转录水平上抑制基因表达，并且在基因组作图、基因克隆、序列特异性药物递送以及基因表达的选择性调控方面也很有用。