Lanfranco L, Wyss P, Marzachí C, Bonfante P
Centro di Studio sulla Micologia del Terreno del CNR, Torino, Italy.
FEMS Microbiol Lett. 1993 Dec 15;114(3):245-51. doi: 10.1111/j.1574-6968.1993.tb06581.x.
The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), consistently appearing when genomic DNA was amplified with an aspecific primer (OPA-18), was found to be a good marker for Tuber magnatum, and was used as a probe in Southern hybridization experiments. The specificity of the results suggests that this probe may be useful in developing specific primers for PCR amplifications.
采用随机扩增多态性DNA（RAPD）技术开发DNA探针，用于鉴定块菌属的外生菌根真菌。RAPD指纹图谱显示种间变异程度高，种内变异程度低。用一条非特异性引物（OPA - 18）扩增基因组DNA时，始终出现的一条带（约1.5 kb）被发现是意大利白块菌的良好标记，并用作Southern杂交实验中的探针。结果的特异性表明该探针可能有助于开发用于PCR扩增的特异性引物。
