Bertini Luana, Rossi Ismaela, Zambonelli Alessandra, Amicucci Antonella, Sacchi Achille, Cecchini Matteo, Gregori Gianluigi, Stocchi Vilberto
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Carlo Bo, Via Saffi 2, 61029 Urbino (PU), Italy.
Microbiol Res. 2006;161(1):59-64. doi: 10.1016/j.micres.2005.06.003. Epub 2005 Jul 28.
Tuber ectomycorrhizae in a Tuber magnatum "truffière", located in Central Italy, were studied using molecular methods. Specifically, RFLP-ITS analyses, ITS sequencing and specific probes hybridization were used to identify 335 Tuber-like ectomycorrhizal morphotypes. Molecular identification was possible even when distinct morphological characteristics were lacking. For the first time, T. magnatum ectomycorrhizae and other coexisting Tuber species collected in the field were analysed using molecular tools for unambiguous identification. Although the "truffière" under investigation yields good harvests of T. magnatum fruiting bodies, the percentage of T. magnatum ectomycorrhizae found was very low (less than 4.4% of the 335 root tips analysed), whereas the percentages of Tuber maculatum and Tuber rufum were considerably higher (48.9% and 19.0%, respectively).
采用分子方法对位于意大利中部的白松露“松露园”中的块菌外生菌根进行了研究。具体而言，利用限制性片段长度多态性-内转录间隔区（RFLP-ITS）分析、ITS测序和特异性探针杂交来鉴定335种块菌状外生菌根形态型。即使缺乏明显的形态特征，分子鉴定也是可行的。首次使用分子工具对在野外采集的白块菌外生菌根和其他共存的块菌物种进行分析，以进行明确鉴定。尽管所研究的“松露园”白松露子实体产量颇丰，但所发现的白块菌外生菌根的比例非常低（在所分析的335个根尖中不到4.4%），而疣孢块菌和红块菌的比例则要高得多（分别为48.9%和19.0%）。
