McHugh T D, Gbewonyo A, Johnson J D, Holliman R E, Butcher P D
Department of Medical Microbiology, St. George's Hospital Medical School, London, UK.
FEMS Microbiol Lett. 1993 Dec 15;114(3):325-32. doi: 10.1111/j.1574-6968.1993.tb06593.x.
Toxoplasma gondii tissue cyst reactivation is a major pathogenic mechanism in ocular toxoplasmosis, disease associated with AIDS and organ transplantation. The mechanisms associated with cyst formation and reactivation have not been elucidated. The complexity of studying these issues in animal models has led to the development of in vitro tissue culture strategies for cyst formation. In the present study we have adopted the human embryonic lung fibroblast (HEL) as the host cell and have compared the cyst forming abilities of eight clinical isolates. We describe by transmission electron microscopy and quantitative light microscopy the development of cysts in vitro. The numbers of in vitro cysts increased with time for all isolates. Cyst cultures were stabilised by manipulation of the free parasite load, an observation not previously recorded. Thus, in this paper we describe a viable model for the analysis of the mechanisms of Toxoplasma cyst development.
弓形虫组织囊肿再激活是眼弓形虫病、与艾滋病和器官移植相关疾病的主要致病机制。与囊肿形成和再激活相关的机制尚未阐明。在动物模型中研究这些问题的复杂性导致了用于囊肿形成的体外组织培养策略的发展。在本研究中，我们采用人胚肺成纤维细胞（HEL）作为宿主细胞，并比较了八个临床分离株的囊肿形成能力。我们通过透射电子显微镜和定量光学显微镜描述了体外囊肿的发育情况。所有分离株的体外囊肿数量均随时间增加。通过控制游离寄生虫负荷使囊肿培养物稳定，这一观察结果此前未被记录。因此，在本文中我们描述了一个用于分析弓形虫囊肿发育机制的可行模型。
