Ried T, Arnold N, Ward D C, Wienberg J
Yale University School of Medicine, Department of Genetics, New Haven, Connecticut 06510.
Genomics. 1993 Nov;18(2):381-6. doi: 10.1006/geno.1993.1479.
A molecular cytogenetic approach that facilitates high-resolution comparative mapping of defined human genes in different primate species is presented. Fluorescence in situ hybridization and digital imaging microscopy were used to visualize human DNA probes on simultaneously banded or "painted" metaphase chromosomes of great apes (Pan troglodytes, Gorilla gorilla, Pongo pygmaeus), hylobatids (Hylobates lar and Hylobates syndactylus), and Old World monkeys (Macaca fuscata and Cercopithecus aethiops). Using a series of DNA probes, chromosomal rearrangements in the karyotypes of primates were readily detected at the molecular cytogenetic level. This approach should contribute considerably to the understanding of primate phylogeny and evolution.
本文介绍了一种分子细胞遗传学方法，该方法有助于在不同灵长类物种中对特定人类基因进行高分辨率比较定位。利用荧光原位杂交和数字成像显微镜技术，在大猩猩（黑猩猩、大猩猩、猩猩）、长臂猿（白掌长臂猿和合趾猿）以及旧大陆猴（猕猴和非洲绿猴）同时显带或“涂色”的中期染色体上观察人类DNA探针。通过一系列DNA探针，在分子细胞遗传学水平上很容易检测到灵长类核型中的染色体重排。这种方法将极大地有助于理解灵长类系统发育和进化。
