Müller S, Koehler U, Weinberg J, Marzella R, Finelli P, Antonacci R, Rocchi M, Archidiacono N
Institut für Anthropologie und Humangenetik, Universität München, Germany.
Chromosome Res. 1996 Jan;4(1):38-42. doi: 10.1007/BF02254943.
We have used Alu polymerase chain reaction generated probes from rearranged human/rodent somatic cell hybrids for fluorescence in situ hybridization and comparative mapping of some intrachromosomal changes in the karyotypes of great apes (Pan troglodytes, P. paniscus, Gorilla gorilla, Pongo pygmaeus), a gibbon (Hylobates lar), and an Old World monkey (Macaca fuscata). Probes containing chromosomes 2 and 18 fragments confirmed inversions already suggested by the banding pattern of great ape homologues. However, a chromosome 3 fragment showed complex rearrangements in the gibbon and macaque karyotype which were previously not well defined from banding. 'Subchromosomal painting' will allow the identification of intrachromosomal changes on the basis of DNA homology and provides a powerful method to study karyological and genomic evolution.
我们利用经Alu聚合酶链式反应生成的、来自重排的人/啮齿动物体细胞杂种的探针，进行荧光原位杂交，并对大猩猩（黑猩猩、倭黑猩猩、大猩猩、猩猩）、长臂猿和旧大陆猴（猕猴）核型中的一些染色体内变化进行比较定位。含有2号和18号染色体片段的探针证实了大猩猩同源染色体带型已暗示的倒位。然而，一个3号染色体片段在长臂猿和猕猴的核型中显示出复杂的重排，这些重排以前通过带型不能很好地界定。“亚染色体涂染”将允许基于DNA同源性鉴定染色体内变化，并提供一种研究核型学和基因组进化的有力方法。
