Koch-Nolte F, Haag F, Kühl M, van Heyningen V, Hoovers J, Grzeschik K H, Singh S, Thiele H G
Department of Immunology, University Hospital, Hamburg, Federal Republic of Germany.
Genomics. 1993 Nov;18(2):404-6. doi: 10.1006/geno.1993.1484.
RT6 is a T cell membrane protein that has attracted interest because a defect in RT6 expression is associated with susceptibility to autoimmune type I diabetes in DP-BB rats and NOD mice. Using PCR screening of human/rodent somatic cell hybrids and fluorescence in situ hybridization, we have determined that the gene for the human RT6 homologue is located at 11q13, centromeric to the gene for tyrosinase (TYR, albino locus) and telomeric to that for fibroblast growth factor 4 (FGF4). The data suggest that the human RT6 gene constitutes a new linkage group with TYR and the gene for olfactory marker protein (OMP) on 11q, which has a counterpart in mouse chromosome 7. Thus, in the human, the RT6 locus is dissociated from the hemoglobin beta chain locus (HBB) and its neighboring conserved linkage group at 11p15, in contrast to the mouse, in which RT6 shows a tighter linkage to Hbb than to Tyr. The results support the conclusion that there has been considerable intrachromosomal reshuffling of linked genes since the divergence of primates and rodents.
RT6是一种T细胞膜蛋白，因其表达缺陷与DP - BB大鼠和NOD小鼠的自身免疫性I型糖尿病易感性相关而备受关注。通过对人/啮齿动物体细胞杂种进行PCR筛选和荧光原位杂交，我们确定人类RT6同源基因位于11q13，在酪氨酸酶（TYR，白化病基因座）基因的着丝粒侧，成纤维细胞生长因子4（FGF4）基因的端粒侧。数据表明，人类RT6基因与11q上的TYR和嗅觉标记蛋白（OMP）基因构成一个新的连锁群，在小鼠7号染色体上有对应物。因此，在人类中，RT6基因座与11p15的血红蛋白β链基因座（HBB）及其相邻的保守连锁群分离，而在小鼠中，RT6与Hbb的连锁比与Tyr更紧密。这些结果支持了自灵长类动物和啮齿动物分化以来，连锁基因在染色体内发生了大量重排的结论。
