Thiermann A B
Am J Vet Res. 1980 Oct;41(10):1659-61.
Among 433 stray dogs in Detroit, Michigan, surveyed for leptospiral agglutinins, 164 (37.8%) had significant titers to 1 or more leptospiral serotypes; from 123 suburban stray dogs tested, 23 (18.7%) had significant titers. The use of 2-mercaptoethanol on positive serum samples was helpful in identifying acute from chronic phase titers. Cross-reaction patterns also were clarified by the use of 2-mercaptoethanol. Cultural isolation of leptospires from dog urine was unsuccessful unless the urine had a pH from 6.6 to 8.0. Oral administration of sodium bicarbonate to alkalinize dog's urine resulted in the isolation of leptospires from dogs usually voiding acidic urine. Leptospira icterohaemorrhagiae was the most common serotype in this dog population by serologic techniques and was the only one isolated in culture.
在密歇根州底特律市对433只流浪狗进行钩端螺旋体凝集素检测，其中164只（37.8%）对1种或更多种钩端螺旋体血清型有显著效价；在123只郊区流浪狗的检测中，23只（18.7%）有显著效价。对阳性血清样本使用2-巯基乙醇有助于区分急性期和慢性期效价。使用2-巯基乙醇也能明确交叉反应模式。除非尿液pH值在6.6至8.0之间，否则从狗尿液中进行钩端螺旋体的培养分离是不成功的。口服碳酸氢钠使狗尿液碱化后，通常能从排出酸性尿液的狗中分离出钩端螺旋体。通过血清学技术，出血性黄疸钩端螺旋体是该犬群中最常见的血清型，也是唯一在培养中分离出的血清型。
