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编码噬菌体Qβ宿主因子的大肠杆菌hfq基因的调控

Regulation of the Escherichia coli hfq gene encoding the host factor for phage Q beta.

作者信息

Kajitani M, Kato A, Wada A, Inokuchi Y, Ishihama A

机构信息

Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.

出版信息

J Bacteriol. 1994 Jan;176(2):531-4. doi: 10.1128/jb.176.2.531-534.1994.

DOI:10.1128/jb.176.2.531-534.1994
PMID:8288550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205081/
Abstract

The host factor (HF-I) for phage Q beta RNA replication is a small protein of 102 amino acid residues encoded by the hfq gene at 94.8 min on the Escherichia coli chromosome. The synthesis rate of HF-I at the exponential-growth phase is higher than at the stationary phase, and it increases concomitantly with the increase in cell growth rate. The intracellular level of HF-I is about 30,000 to 60,000 molecules per cell, the majority being associated with ribosomes as one of the salt wash proteins. Taken together, we suggest that HF-I is one of the growth-related proteins.

摘要

噬菌体Qβ RNA复制的宿主因子(HF-I)是一种由102个氨基酸残基组成的小蛋白,由大肠杆菌染色体上94.8分钟处的hfq基因编码。HF-I在指数生长期的合成速率高于稳定期,并且随着细胞生长速率的增加而同步增加。HF-I的细胞内水平约为每个细胞30,000至60,000个分子,其中大部分作为盐洗蛋白之一与核糖体结合。综上所述,我们认为HF-I是一种与生长相关的蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4075/205081/13a796ece40f/jbacter00020-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4075/205081/13a796ece40f/jbacter00020-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4075/205081/13a796ece40f/jbacter00020-0277-a.jpg

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