噬菌体Qβ宿主因子基因的鉴定与序列测定
Identification and sequence determination of the host factor gene for bacteriophage Q beta.
作者信息
Kajitani M, Ishihama A
机构信息
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
出版信息
Nucleic Acids Res. 1991 Mar 11;19(5):1063-6. doi: 10.1093/nar/19.5.1063.
Abstract
The host factor (HF-I) required for phage Q beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues.
摘要
噬菌体Qβ RNA指导合成互补负链RNA所需的宿主因子（HF-I）从噬菌体感染的大肠杆菌细胞中纯化至同质。基于HF-I的部分氨基酸序列设计合成探针，克隆了编码HF-I的hfq基因，并将其定位在大肠杆菌染色体上94.8分钟处，位于参与2-甲硫基-N6-（异戊基）-腺苷（ms2i6A）tRNA修饰的miaA基因下游。对克隆的hfq基因进行序列测定表明，HF-I是一种分子量为11166的小蛋白，由102个氨基酸残基组成。
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