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通过GDP解离抑制剂从高尔基体膜上去除Rab GTP结合蛋白会抑制高尔基体堆叠中的顺式间运输。

Removal of Rab GTP-binding proteins from Golgi membranes by GDP dissociation inhibitor inhibits inter-cisternal transport in the Golgi stacks.

作者信息

Elazar Z, Mayer T, Rothman J E

机构信息

Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

出版信息

J Biol Chem. 1994 Jan 14;269(2):794-7.

PMID:8288626
Abstract

Rab proteins are a family of Ras-like GTPases involved in intracellular membrane traffic. Rab GDI, a cytosolic protein which inhibits the dissociation of GDP from various Rab proteins, is required to maintain a pool of Rab proteins in the cytosol. We describe the purification of a cytosolic factor from bovine liver that inhibits intra-cisternal transport between the Golgi stacks. We identify this factor as a Rab GDI. Half-maximal inhibition of transport was observed in the presence of the same concentration of GDI that is required for removal of Rab proteins from the Golgi.

摘要

Rab蛋白是一类参与细胞内膜运输的Ras样GTP酶。Rab GDI是一种胞质蛋白，可抑制GDP从各种Rab蛋白上解离，它对于在胞质溶胶中维持Rab蛋白库是必需的。我们描述了从牛肝脏中纯化出一种抑制高尔基体堆叠之间顺式内运输的胞质因子。我们鉴定该因子为Rab GDI。在存在与从高尔基体去除Rab蛋白所需相同浓度的GDI时，观察到运输的半数最大抑制。

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