Mason H D, Mannaerts B, de Leeuw R, Willis D S, Franks S
Department of Obstetrics and Gynaecology, Imperial College of Science Technology and Medicine, St Mary's Hospital Medical School, London, UK.
Hum Reprod. 1993 Nov;8(11):1823-7. doi: 10.1093/oxfordjournals.humrep.a137941.
The effects of recombinant human follicle stimulating hormone (rFSH; Org 32489) have been examined in human granulosa cells from ovaries obtained from women with spontaneous menses. In the first series of experiments the actions of rFSH on production of oestradiol and progesterone were compared with those of urinary-derived gonadotrophins. Recombinant FSH induced dose-dependent increases in production of both oestradiol and progesterone which were similar to the effects of 'pure' FSH (Metrodin) and the International Standard IS 71/223. In further studies, the actions of rFSH on oestradiol production by individual preovulatory follicles were investigated; rFSH increased oestradiol accumulation from cells obtained from follicles before the luteinizing hormone (LH) surge. In contrast, rFSH inhibited oestradiol production by granulosa cells derived from a follicle after the onset of the LH surge, whereas the gonadotrophic action of growth hormone was maintained. Following preliminary reports of the in-vivo effects of rFSH in women, these findings provide further validation of the efficacy of rFSH in the human ovary. The results of studies of the preovulatory follicle illustrate the experimental importance of the availability of recombinant preparations of pure gonadotrophins, produced by recombinant technology, in the understanding of human ovarian function.
重组人促卵泡激素（rFSH；Org 32489）对来自有自然月经周期女性卵巢的人颗粒细胞的作用已得到研究。在第一系列实验中，将rFSH对雌二醇和孕酮产生的作用与尿源性促性腺激素的作用进行了比较。重组FSH诱导雌二醇和孕酮产生呈剂量依赖性增加，这与“纯”FSH（Metrodin）和国际标准品IS 71/223的作用相似。在进一步研究中，研究了rFSH对单个排卵前卵泡雌二醇产生的作用；rFSH增加了在促黄体生成素（LH）峰前从卵泡获得的细胞中雌二醇的积累。相比之下，rFSH抑制了LH峰开始后从卵泡衍生的颗粒细胞的雌二醇产生，而生长激素的促性腺作用得以维持。继rFSH对女性体内作用的初步报告之后，这些发现进一步证实了rFSH在人卵巢中的功效。对排卵前卵泡的研究结果说明了通过重组技术生产的纯促性腺激素重组制剂在理解人类卵巢功能方面的实验重要性。
