Sugane K, Sun S H
Department of Parasitology, Shinshu University School of Medicine, Nagano prefecture, Japan.
J Immunol Methods. 1994 Jan 12;168(1):55-60. doi: 10.1016/0022-1759(94)90209-7.
An enzyme-linked immunosorbent assay (ELISA) using antigenic beta-galactosidase-helminth derived recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human helminthiasis. Helminth-infected human sera reacted strongly with FP that was immobilized with anti-beta-galactosidase monoclonal antibody on microplates. However, FP did not react with sera from patients with other helminthiases. In detection of anti-helminth IgG antibody, the present ELISA system using FP and anti-beta-galactosidase monoclonal antibody was highly specific compared to that using biochemically extracted soluble antigens.
