Sun S, Sugane K
Department of Parasitology, Faculty of Medicine, Shinshu University, Nagano Prefecture, Japan.
J Helminthol. 1992 Sep;66(3):220-6. doi: 10.1017/s0022149x00014590.
An enzyme-linked immunosorbent assay (ELISA) using antigenic beta-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-beta-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.
使用通过重组DNA技术获得的抗原性β-半乳糖苷酶-犬恶丝虫重组融合蛋白（FP）的酶联免疫吸附测定（ELISA）为人类恶丝虫病提供了一种有用的诊断工具。感染犬恶丝虫的人血清与用抗β-半乳糖苷酶单克隆抗体固定在微孔板上的FP发生强烈反应。然而，FP与其他丝虫病患者的血清不发生反应。在检测抗犬恶丝虫IgG抗体时，与使用粗制虫体抗原的ELISA相比，使用FP的ELISA具有更高的敏感性和特异性。
