• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过液氮冷却铜块进行快速冷冻的经验性测定冷冻时间。

Empirically determined freezing time for quick-freezing with a liquid-nitrogen-cooled copper block.

作者信息

Baatsen P H

机构信息

Departement de Physiologie générale, Université Catholique de Louvain, Brussels, Belgium.

出版信息

J Microsc. 1993 Oct;172(Pt 1):71-9. doi: 10.1111/j.1365-2818.1993.tb03395.x.

DOI:10.1111/j.1365-2818.1993.tb03395.x
PMID:8289229
Abstract

A method is presented to determine freezing time empirically. The method is based on determining the amount of stretch of a skinned muscle fibre while it is being frozen. Freezing time, as determined with this method lies in between 0.5 and 1.5 ms.

摘要

提出了一种凭经验确定冷冻时间的方法。该方法基于在冷冻去皮肌纤维时确定其拉伸量。用这种方法确定的冷冻时间在0.5到1.5毫秒之间。

相似文献

1
Empirically determined freezing time for quick-freezing with a liquid-nitrogen-cooled copper block.通过液氮冷却铜块进行快速冷冻的经验性测定冷冻时间。
J Microsc. 1993 Oct;172(Pt 1):71-9. doi: 10.1111/j.1365-2818.1993.tb03395.x.
2
A method for quick-freezing live muscles at known instants during contraction with simultaneous recording of mechanical tension.
J Microsc. 1988 Aug;151(Pt 2):81-102. doi: 10.1111/j.1365-2818.1988.tb04616.x.
3
The quick-freezing of single intact skeletal muscle fibers at known time intervals following electrical stimulation.在电刺激后按已知时间间隔对单个完整骨骼肌纤维进行速冻。
Scan Electron Microsc. 1986(Pt 1):309-28.
4
Series elasticity in frog sartorius muscle subjected to stretch-shortening cycles.
J Biomech. 1990;23(2):121-6. doi: 10.1016/0021-9290(90)90346-5.
5
Cardiac muscle following quick-freezing: preservation of in vivo ultrastructure and geometry with special emphasis on intercellular clefts in the intact frog heart.
J Mol Cell Cardiol. 1988 Apr;20(4):285-302. doi: 10.1016/s0022-2828(88)80063-4.
6
Ultrastructure of skeletal muscle fibers studied by a plunge quick freezing method: myofilament lengths.采用骤冷速冻法研究骨骼肌纤维的超微结构：肌丝长度
Biophys J. 1994 Jul;67(1):283-92. doi: 10.1016/S0006-3495(94)80479-5.
7
The effect of a small stretch on the latency relaxation and the short-range elastic stiffness in isolated frog muscle fibres.小幅度拉伸对离体青蛙肌纤维延迟松弛和短程弹性刚度的影响。
Acta Physiol Scand. 1981 Jun;112(2):121-8. doi: 10.1111/j.1748-1716.1981.tb06794.x.
8
[Method for direct determination of the rotational symmetry of thick muscle filaments by digital image processing].
Acta Cient Venez. 1991;42(2):59-63.
9
Short-range elasticity after tetanic stimulation in single muscle fibres of the frog.青蛙单根肌纤维强直刺激后的短程弹性
Acta Physiol Scand. 1982 Apr;114(4):487-95. doi: 10.1111/j.1748-1716.1982.tb07016.x.
10
Pulsed NMR studies of water in striated muscle. II. Spin-lattice relaxation times and the dynamics of non-freezing fraction of water.
Biochim Biophys Acta. 1973 Mar 30;304(1):56-64. doi: 10.1016/0304-4165(73)90114-1.

引用本文的文献

1
Hepatic secretion of phospholipid vesicles in the mouse critically depends on mdr2 or MDR3 P-glycoprotein expression. Visualization by electron microscopy.小鼠肝脏中磷脂囊泡的分泌严重依赖于mdr2或MDR3 P-糖蛋白的表达。通过电子显微镜观察。
J Clin Invest. 1997 Nov 15;100(10):2562-7. doi: 10.1172/JCI119799.