Curran J F, Gross B L
Department of Biology, Wake Forest University, Winston-Salem, NC 27109.
J Mol Biol. 1994 Jan 7;235(1):389-95. doi: 10.1016/s0022-2836(05)80046-4.
It has been suggested that a triplet repeated pattern found in coding sequences, the G-nonG-N or GHN phase bias, serves a framing function during protein synthesis. To test this idea, the framing characteristics of a highly GHN biased sequence are examined. No effects on reading frame maintenance are observed despite the use of sensitive frameshift assays. Specifically, first the GHN phase is not more accurate than the alternative overlapping phases (i.e., HNG and NGH). Second, ribosomes do not exhibit any significant tendency to slip from the alternative frames into the GHN pahse. In addition, examination of Escherichia coli programmed frameshift sites does not support roles for GHN phase bias in programmed frameshifting. Framing functions for GHN phase bias, if they occur at all, must be extremely limited.
有人提出，在编码序列中发现的一种三联体重复模式，即G-非G-N或GHN相位偏倚，在蛋白质合成过程中起框架作用。为了验证这一想法，研究了一个高度偏向GHN的序列的框架特征。尽管使用了灵敏的移码检测方法，但未观察到对阅读框维持的影响。具体而言，首先，GHN相位并不比其他重叠相位（即HNG和NGH）更准确。其次，核糖体没有表现出从其他阅读框滑入GHN相位的任何明显趋势。此外，对大肠杆菌编程移码位点的研究不支持GHN相位偏倚在编程移码中的作用。如果GHN相位偏倚确实具有框架功能，那么其功能必定极其有限。
