白色念珠菌磷酸甘露糖异构酶的结晶及初步X射线分析

Crystallization and preliminary X-ray analysis of Candida albicans phosphomannose isomerase.

作者信息

Tolley S, Davies G, Hubbard R E, Smith D J, Proudfoot A E, Payton M A, Cleasby A, Wonacott A, Wells T N

机构信息

epartment of Chemistry, University of York, Heslington, U.K.

出版信息

J Mol Biol. 1994 Apr 1;237(3):349-50. doi: 10.1006/jmbi.1994.1235.

DOI:10.1006/jmbi.1994.1235

PMID:8145246

Abstract

Crystals of recombinant phosphomannose isomerase from Candida albicans have been obtained in a form suitable for X-ray diffraction analysis. The enzyme plays a key role in the biosynthesis of the mannan component of the fungal cell wall. It crystallizes in monoclinic space group C2, with cell dimensions a = 124.9 A, b = 52.9 A, c = 85.9 A and beta = 127.4 degrees. The crystals diffract to Bragg spacings beyond 1.7 A, native data have been collected to 2.4 A and a search for heavy-metal derivatives is in progress. The asymmetric unit contains one molecule of the enzyme (M(r) approximately 49,000) with a Vm of 2.3 A3/Da.

摘要

已获得白色念珠菌重组磷酸甘露糖异构酶的晶体，其形式适合进行X射线衍射分析。该酶在真菌细胞壁甘露聚糖成分的生物合成中起关键作用。它以单斜晶系空间群C2结晶，晶胞参数为a = 124.9 Å，b = 52.9 Å，c = 85.9 Å，β = 127.4°。这些晶体的衍射极限达到布拉格间距超过1.7 Å，已收集到2.4 Å的天然数据，目前正在寻找重金属衍生物。不对称单元包含一个酶分子（相对分子质量约为49,000），Vm为2.3 Å³/Da。