David-Dufilho M, Pernollet M G, Morris M, Astarie-Dekequer C, Devynck M A
Pharmacology, URA CNRS 1482, CHU Necker, Paris, France.
Life Sci. 1994;54(4):267-74. doi: 10.1016/0024-3205(94)00816-7.
Cytosolic Ca2+ concentration ([Ca2+]i) and 45Ca2+ influx were investigated in erythrocytes from conscious spontaneously hypertensive rats (SHR) and their normotensive controls Wistar-Kyoto (WKY). [Ca2+]i was evaluated with fura-2 and intra- and extra-cellular calibration parameters were compared. Irrespective of the calibration parameters used, erythrocyte [Ca2+]i was always significantly higher in SHR than in WKY and Wistar rats (by 25 and 40%, p < 0.01 and 0.001). A rise of the external Ca2+ concentration from 1 to 2 mmol/l increased less [Ca2+]i in SHR than in WKY erythrocytes (17 vs 37%, p < 0.01). SHR erythrocytes incorporated more 45Ca2+ than those from WKY, with an initial rate of 45Ca2+ uptake higher by 57% than that of WKY erythrocytes (p < 0.05). Vanadate ions, after corrections of their quenching effect on red cell and fura-2 fluorescence signals, increased [Ca2+]i by 19% in WKY erythrocytes (p = 0.05), but did not modify the SHR values. They also increased 45Ca2+ accumulation and the initial rate of 45Ca2+ influx in WKY erythrocytes only (p < 0.01). This study indicates that, when compared to WKY rats, erythrocytes from SHR are characterized by higher [Ca2+]i values, higher initial rate of Ca2+ influx and low sensitivity to vanadate ions.
研究了清醒状态下自发性高血压大鼠（SHR）及其正常血压对照Wistar-Kyoto（WKY）红细胞中的胞质Ca2+浓度（[Ca2+]i）和45Ca2+内流。用fura-2评估[Ca2+]i，并比较细胞内和细胞外校准参数。无论使用何种校准参数，SHR红细胞中的[Ca2+]i总是显著高于WKY和Wistar大鼠（分别高25%和40%，p<0.01和0.001）。外部Ca2+浓度从1mmol/l升至2mmol/l时，SHR红细胞中[Ca2+]i的升高幅度小于WKY红细胞（17%对37%，p<0.01）。SHR红细胞比WKY红细胞摄取更多的45Ca2+，45Ca2+摄取的初始速率比WKY红细胞高57%（p<0.05）。钒离子在校正其对红细胞和fura-2荧光信号的淬灭作用后，使WKY红细胞中的[Ca2+]i升高了19%（p=0.05），但对SHR的值没有影响。它们还仅增加了WKY红细胞中45Ca2+的积累和45Ca2+内流的初始速率（p<0.01）。这项研究表明，与WKY大鼠相比，SHR红细胞的特点是[Ca2+]i值较高、Ca2+内流初始速率较高以及对钒离子的敏感性较低。
