Wang S J, Lin W Y, Kao C H, Hsu C Y, Lee T W, Chen M N, Ting G, Shen L H
Department of Nuclear Medicine, Veterans General Hospital, Taichung, Taiwan.
Nucl Med Commun. 1993 Nov;14(11):1030-4. doi: 10.1097/00006231-199311000-00015.
Radiolabelled human immunoglobulin (HIG) scintigraphy has been recognized as a reliable modality for the localization and evaluation of pyogenic infection in humans. Four methods of labelling HIG with 99Tcm developed by the Institute of Nuclear Energy Research of Taiwan are: (1) using ascorbic acid (ASC) for direct labelling of HIG (ASC-HIG); (2) using diethylenetriamine pentaacetate (DTPA) as a bifunctional chelate (DTPA-HIG); (3) using 2-iminothiolane (IM) for modified protein (IM-HIG); (4) using the avidin-biotin system (AVI-BIO-HIG). Our study assessed these four preparations in the scintigraphic detection of infections. Staphylococcus aureus was injected intramuscularly into the left thighs of rabbits. Two days later, when active inflammation developed, 5 mCi 99Tcm-HIG was given intravenously; scintigraphy was performed at 1, 4 and 18 h. The accumulation of 99Tcm DTPA-HIG was the most prominent among the four preparations at the site of infection. It is concluded that 99Tcm labelling of HIG using DTPA as a bifunctional chelate may be the optimal labelling method for detecting infection.
放射性标记的人免疫球蛋白（HIG）闪烁扫描术已被公认为是一种用于定位和评估人类化脓性感染的可靠方法。台湾核能研究所开发的四种用99锝标记HIG的方法是：（1）使用抗坏血酸（ASC）直接标记HIG（ASC-HIG）；（2）使用二乙烯三胺五乙酸（DTPA）作为双功能螯合剂（DTPA-HIG）；（3）使用2-亚氨基硫醇（IM）修饰蛋白质（IM-HIG）；（4）使用抗生物素蛋白-生物素系统（AVI-BIO-HIG）。我们的研究评估了这四种制剂在感染闪烁扫描检测中的效果。将金黄色葡萄球菌肌肉注射到兔子的左大腿。两天后，当出现活动性炎症时，静脉注射5毫居里的99锝-HIG；在1、4和18小时进行闪烁扫描。在感染部位，99锝-DTPA-HIG在这四种制剂中的聚集最为显著。结论是，使用DTPA作为双功能螯合剂对HIG进行99锝标记可能是检测感染的最佳标记方法。
