de Bois M H, Welling M, Verwey C L, de Vries E, Pauwels E K, Breedveld F C, Tak P P
Department of Rheumatology, University Hospital Leiden, The Netherlands.
Nucl Med Commun. 1996 Jan;17(1):54-9. doi: 10.1097/00006231-199601000-00010.
Our objective was to investigate the mechanism of accumulation of 99Tcm-labelled non-specific polyclonal human immunoglobulin (99Tcm-HIG) in inflamed synovial tissue (ST) in an experimental animal model of arthritis. Following 99Tcm-HIG scintigraphy, the in vivo localization of 99Tcm-HIG in the ST of knee joints of rats with adjuvant arthritis was studied using immunohistochemical techniques. In addition, the in vitro binding of 99Tcm-HIG to extracellular matrix proteins was analysed by means of immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). After 99Tcm-HIG scintigraphy, 99Tcm-HI was detected in the ST of rats with adjuvant arthritis. 99Tcm-HIG was diffusely distributed and not bound to cells. In vitro incubation of 99Tcm-HIG on the ST of rats with adjuvant arthritis revealed binding of 99Tcm-HIG to inflamed, but not to non-inflamed, ST. In addition, specific binding of 99Tcm-HIG to fibronectin, fibrin, collagen type I and III was demonstrated by ELISA. We conclude that the accumulation of 99Tcm-HIG in inflamed ST can be explained by the binding of 99Tcm-HIG to extracellular matrix proteins.
我们的目的是在关节炎实验动物模型中研究99锝标记的非特异性多克隆人免疫球蛋白（99锝-人免疫球蛋白）在炎症滑膜组织（ST）中蓄积的机制。在进行99锝-人免疫球蛋白闪烁扫描后，采用免疫组化技术研究99锝-人免疫球蛋白在佐剂性关节炎大鼠膝关节滑膜组织中的体内定位。此外，通过免疫组化和酶联免疫吸附测定（ELISA）分析99锝-人免疫球蛋白与细胞外基质蛋白的体外结合情况。在99锝-人免疫球蛋白闪烁扫描后，在佐剂性关节炎大鼠的滑膜组织中检测到99锝-人免疫球蛋白。99锝-人免疫球蛋白呈弥漫性分布，不与细胞结合。在佐剂性关节炎大鼠的滑膜组织上进行99锝-人免疫球蛋白的体外孵育，结果显示99锝-人免疫球蛋白与炎症滑膜组织结合，而不与非炎症滑膜组织结合。此外，ELISA证实99锝-人免疫球蛋白与纤连蛋白、纤维蛋白、Ⅰ型和Ⅲ型胶原存在特异性结合。我们得出结论，99锝-人免疫球蛋白在炎症滑膜组织中的蓄积可通过99锝-人免疫球蛋白与细胞外基质蛋白的结合来解释。
